Silver Nanocubes as Electrochemical Labels for Bioassays
| Autor: | Nicole E. Pollok, Charlie Rabin, Leilani Smith, Yi Peng, Charuksha Walgama, Richard M. Crooks, Ian Richards |
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| Rok vydání: | 2021 |
| Předmět: |
Fluid Flow and Transfer Processes
Detection limit Silver Materials science Process Chemistry and Technology 010401 analytical chemistry Inorganic chemistry Metal Nanoparticles Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences Article Silver nanoparticle 0104 chemical sciences Anodic stripping voltammetry Galvanic cell Biological Assay 0210 nano-technology Electrodes Instrumentation Order of magnitude |
| Zdroj: | ACS Sens |
| ISSN: | 2379-3694 |
| Popis: | Here we report on the use of 40 ± 4 nm silver nanocubes (AgNCs) as electrochemical labels in bioassays. The model metalloimmunoassay combines galvanic exchange (GE) and anodic stripping voltammetry (ASV). The results show that a lower limit of detection is achieved by simply changing the shape of the Ag label yielding improved GE with AgNCs when compared to GE with spherical nanoparticles (NPs). Specifically, during GE between electrogenerated Au(3+) and the Ag labels, a thin shell of Au forms on the surface of the NP. This shell is more porous when GE proceeds on AgNCs compared to spherical AgNPs, and therefore more exchange occurs when using AgNCs. ASV results show that the Ag collection efficiency (AgCE%) is increased by up to ~57% when using AgNCs. When the electrochemical system is fully optimized, the limit of detection is 0.1 pM AgNCs, which is an order of magnitude lower than that for spherical AgNP labels. |
| Databáze: | OpenAIRE |
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