Vibrio harveyi RNA polymerase: purification and resolution from gyrase A
| Autor: | Elana Swartzman, Edward A. Meighen |
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| Rok vydání: | 1992 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data RNA-dependent RNA polymerase RNA polymerase II Biochemistry DNA gyrase chemistry.chemical_compound Bacterial Proteins Transcription (biology) RNA polymerase RNA polymerase I Amino Acid Sequence Molecular Biology Polymerase Vibrio biology Vibrio harveyi DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial Cell Biology biology.organism_classification Molecular biology DNA Topoisomerases Type II chemistry Genes Bacterial Luminescent Measurements biology.protein bacteria Sequence Alignment |
| Zdroj: | Biochemistry and Cell Biology. 70:698-702 |
| ISSN: | 1208-6002 0829-8211 |
| Popis: | RNA polymerase was purified from Vibrio harveyi and found to contain polypeptides (β,β′, α, and σ) closely corresponding to those of the Escherichia coli enzyme. In vitro transcription studies using V. harveyi and E. coli RNA polymerase demonstrated that the purified V. harveyi RNA polymerase is functional and that the two enzymes have the same promoter specificity. Chromatography through a monoQ column was required to remove a 100-kilodalton protein that was present in large amounts and copurified with the RNA polymerase. N-terminal amino acid sequencing showed that the first 18 amino acids of the 100-kilodalton protein shares 78% sequence identity with the A subunit of gyrase or topoisomerase II. The abundance of the gyrase A protein is unprecedented and may be linked to bioluminescence.Key words: Vibrio harveyi, RNA polymerase, gyrase, bioluminescence. |
| Databáze: | OpenAIRE |
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