Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model
| Autor: | Erik Braziulis, Sophie Böttcher-Haberzeth, Luca Pontiggia, Thomas Biedermann, Fabienne Hartmann-Fritsch, Clemens Schiestl, Martin Meuli, Ernst Reichmann |
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| Přispěvatelé: | University of Zurich, Meuli, M |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
Pathology medicine.medical_specialty Swine Human skin 610 Medicine & health Transplantation Autologous Dermal fibroblast Tissue engineering Animals Autologous transplantation Medicine 10220 Clinic for Surgery 2735 Pediatrics Perinatology and Child Health Fibroblast Cell Proliferation Microscopy Confocal Tissue Engineering Epidermis (botany) integumentary system business.industry Graft Survival Dermis Skin Transplantation General Medicine 2746 Surgery Transplantation medicine.anatomical_structure Epidermal Cells Microscopy Fluorescence Models Animal Pediatrics Perinatology and Child Health Self-healing hydrogels Surgery business |
| Popis: | Background: Extended full thickness skin defects still represent a considerable therapeutic challenge as ideal strategies for definitive autologous coverage are still not available. Tissue engineering of whole skin represents an equally attractive and ambitious novel approach. We have recently shown that laboratory-grown human skin analogues with near normal skin anatomy can be successfully transplanted on immuno-incompetent rats. The goal of the present study was to engineer autologous porcine skin grafts for transplantation in a large animal model (pig study=intended preclinical study). Materials and methods: Skin biopsies were taken from the pig's abdomen. Epidermal keratinocytes and dermal fibroblasts were isolated and then expanded on culture dishes. Subsequently, highly concentrated collagen hydrogels and collagen/fibrin hydrogels respectively, both containing dermal fibroblasts, were prepared. Fibroblast survival, proliferation, and morphology were monitored using fluorescent labelling and laser scanning confocal microscopy. Finally, keratinocytes were seeded onto this dermal construct and allowed to proliferate. The resulting in vitro generated porcine skin substitutes were analysed by H&E staining and immunofluorescence. Results: Dermal fibroblast proliferation and survival in pure collagen hydrogels was poor. Also, the cells were mainly round-shaped and they did not develop 3D-networks. In collagen/fibrin hydrogels, dermal fibroblast survival was significantly higher. The cells proliferated well, were spindle-shaped, and formed 3D-networks. When these latter dermal constructs were seeded with keratinocytes, a multilayered and partly stratified epidermis readily developed. Conclusion: This study provides compelling evidence that pig cell-derived skin analogues with near normal skin anatomy can be engineered in vitro. These tissue-engineered skin substitutes are needed to develop a large animal model to establish standardized autologous transplantation procedures for those studies that must be conducted before "skingineering” can eventually be clinically applied |
| Databáze: | OpenAIRE |
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