Mapping of the epitopes of poliovirus type 2 in complex with antibodies
| Autor: | Yves Girerd-Chambaz, Jean-Michel Guigner, Ana A. Arteni, Catherine Vénien-Bryan, Ludovic Bannwarth, Frédéric Ronzon, Catherine Manin |
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| Přispěvatelé: | Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de recherche pour le développement [IRD] : UR206-Centre National de la Recherche Scientifique (CNRS), Sanofi Pasteur [Marcy-l'Étoile, France], Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de recherche pour le développement [IRD] : UR206-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Surface Properties medicine.drug_class [SDV]Life Sciences [q-bio] Immunology Biology Antibodies Viral Monoclonal antibody medicine.disease_cause Epitope Virus Epitopes Immunoglobulin Fab Fragments Polio vaccine Antigen Freezing Image Processing Computer-Assisted medicine Amino Acids Antigens Viral Molecular Biology Poliovirus Cryoelectron Microscopy Virology Molecular biology Vaccines Inactivated Polyclonal antibodies biology.protein Antibody Epitope Mapping |
| Zdroj: | Molecular Immunology Molecular Immunology, 2015, 67 (2), pp.233-239. ⟨10.1016/j.molimm.2015.05.013⟩ Molecular Immunology, Elsevier, 2015, 67 (2), pp.233-239. ⟨10.1016/j.molimm.2015.05.013⟩ |
| ISSN: | 0161-5890 |
| Popis: | International audience; The inactivated polio vaccine (IPV) contains poliovirus (PV) samples that belong to serotypes 1, 2 and 3.All three serotypes contain the D-antigen, which induces protective antibodies. The antigenic structureof PVs consists of at least four different antigenic sites and the D-antigen content represents the combinedactivity of multiple epitopes (Ferguson et al., 1993; Minor, 1990; Minor et al., 1986). The potencyof IPV vaccines is determined by measuring the D-antigen content. Several ELISA methods have beendeveloped using polyclonal or monoclonal antibodies (Mabs) in order to quantify the D-antigen content.Characterization of the epitopes recognized by the different Mabs is crucial to map the entire virus surfaceand ensure the presence of epitopes able to induce neutralizing antibodies. Using a new approachthat we developed to study the interaction between monoclonal antibodies and poliovirus type 2, whichcombines cryo-electron microscopy, image analysis and X-ray crystallography along with identificationof exposed amino acids, we have mapped in 3D the epitope sites recognized by three specific Fabs at thesurface of poliovirus type 2 (PV2) and characterized precisely the antigenic sites for these Fabs. |
| Databáze: | OpenAIRE |
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