Genetically Modified Animal Models
| Autor: | Denise Kinoshita, Lucas Martins Chaible, Marcus A.F. Corat, Maria Lúcia Zaidan Dagli |
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| Rok vydání: | 2017 |
| Předmět: |
Genetics
Transcription activator-like effector nuclease Genetically engineered Transgene Cancer Computational biology Biology medicine.disease Zinc finger nuclease Genetically modified organism Genome editing Conditional gene knockout medicine CRISPR Genetically modified mammal Genetically modified animal |
| Popis: | Genetically modified animals were first created in the 1970s–1980s, and are considered good models of human diseases. In this chapter, the types of genetically engineered mice, like transgenic, knockout, conditional knockout and knock-in animals, are presented. More recently, new methodologies of gene editing have been developed, providing new transgenic animals models, including knockout and knockin models of mice and other animal species. These methodologies include zinc fingers nucleases (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR). In special, CRISPR technique became a very important tool to develop new models for the study of human diseases, in an easier, cheaper and faster way compared with traditional methods. In this chapter, the general techniques on how to obtain these animals are described, and most used genetically modified models for genetic diseases, multifactorial, and polygenic (complex) disorders, neurodegenerative, inflammatory diseases, besides cancer models, are presented. The aim of the chapter is to help investigators to find out the best genetically modified animal models for their studies. |
| Databáze: | OpenAIRE |
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