Novel opioid peptides endomorphin-1 and endomorphin-2 are present in mammalian immune tissues
| Autor: | Sarah J Kaye, Michael S. Harbuz, Allison J Fulford, Freddy M De Bree, David S. Jessop, Glenn N. Major, Toni L. Coventry |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male medicine.medical_specialty Immunology Radioimmunoassay Spleen Thymus Gland Biology Cross Reactions chemistry.chemical_compound Internal medicine medicine Immunology and Allergy Animals Humans Rats Wistar Receptor Opioid peptide Chromatography High Pressure Liquid Thymus extract Oligopeptide Tissue Extracts Immune Sera Endomorphin-1 Middle Aged Rats medicine.anatomical_structure Endocrinology Neurology chemistry Immune System Female Neurology (clinical) Endomorphin Oligopeptides |
| Zdroj: | Journal of neuroimmunology. 106(1-2) |
| ISSN: | 0165-5728 |
| Popis: | Endomorphin (EM)-1 and EM-2 are opioid tetrapeptides, reported within the central nervous system, which have very high specificity and affinity for the mu-opioid receptor. We have used newly developed and well-characterised radioimmunoassays (RIAs) in combination with reversed-phase high-performance liquid chromatography (HPLC) to detect EM-1 and EM-2 immunoreactivity (ir) in rat immune tissues. Endomorphins were detectable in extracts of rat spleen (total EM-1-ir/spleen: 440+/-73 pg, mean+/-SEM, a=group of eight rats; EM-2-ir: 150+/-12 pg) and thymus (EM-1-ir: 152+/-18 pg, mean+/-SEM n=8; EM-2-ir: 156+/-28 pg). EM-2-ir was detectable in extracts of human spleen (338+/-196 pg/g tissue, n=3). Multiple peaks of EM-1-ir and EM-2-ir were observed in rat spleen and thymus extracts, and multiple peaks of EM-2-ir were observed in extracts of human spleen, following reversed-phase HPLC and RIAs. This is the first report of endomorphin immunoreactivity in tissues of the rat and human immune systems. |
| Databáze: | OpenAIRE |
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