Characterisation and In Vitro Evaluation of Fenugreek (Trigonella foenum-graecum) Seed Gum as a Potential Prebiotic in Growing Rabbit Nutrition

Autor: Juan José Pascual, L. Ródenas, Taha Najar, Enrique Blas, Jihed Zemzmi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Trigonella
Fenugreek seed
medicine.medical_treatment
rabbit
Prebiotic
Rabbit
PRODUCCION ANIMAL
15.- Proteger
restaurar y promover la utilización sostenible de los ecosistemas terrestres
gestionar de manera sostenible los bosques
combatir la desertificación y detener y revertir la degradación de la tierra
y frenar la pérdida de diversidad biológica
Article
Galactomannan
chemistry.chemical_compound
0404 agricultural biotechnology
Nutrient
digestion in vitro
lcsh:Zoology
medicine
Dry matter
lcsh:QL1-991
Food science
Solubility
fenugreek seed
lcsh:Veterinary medicine
General Veterinary
biology
0402 animal and dairy science
food and beverages
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
040201 dairy & animal science
chemistry
Galactose
galactomannan
prebiotic
Digestion in vitro
lcsh:SF600-1100
Animal Science and Zoology
Fermentation
Zdroj: Animals
Volume 10
Issue 6
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Animals : an Open Access Journal from MDPI
Animals, Vol 10, Iss 1041, p 1041 (2020)
ISSN: 2076-2615
DOI: 10.3390/ani10061041
Popis: [EN] A fenugreek seed gum, extracted fromTrigonella foenum-graecumseeds and rich in galactomannan, was chemically and physically characterised and its prebiotic potential for young rabbits was evaluated in vitro, both as pure fenugreek seed gum and when included up to 20 g/kg in rabbit diets rich in soluble and insoluble fibre. Fenugreek seed gum was resistant to pepsin and pancreatin digestion but was totally fermented by rabbit caecal bacteria. Fenugreek seed gum linear inclusion up to 20 g/kg in diets rich in soluble fibre has led to a reduction in the solubility of some nutrients during in vitro enzymatic phase and an increase in the fermented fraction. Fenugreek seed gum satisfies two essential conditions of a prebiotic: resistance to enzymatic digestion and being totally fermented by caecal bacteria. Some components of soluble fibre appear to have prebiotic effects that can contribute to improving digestive health in post-weaning rabbits. In this work, a fenugreek seed gum (FGS), extracted fromTrigonella foenum-graecumseeds and rich in galactomannan, was characterised. Both the pure FSG and ten substrates obtained by the inclusion of 0, 5, 10, 15 and 20 g/kg of FSG in diets rich in soluble (SF) and insoluble (IF) fibre were evaluated in vitro to determine FSG prebiotic potential for rabbit diets. FSG was rich in total sugars (630 g/kg dry matter), consisting entirely of galactose and mannose in a 1:1 ratio, and a moderate protein content (223 g/kg dry matter). Pure FSG was affected very little by in vitro digestion, as only 145 g/kg of the FSG was dissolved during the enzymatic phase. However, the linear inclusion of FSG up to 20 g/kg in growing rabbit feeds has led to a reduction in the solubility of some nutrients during in vitro enzymatic phase, especially in SF diets. Pure FSG not digested during the enzymatic phase almost completely disappeared during the in vitro fermentation phase, 984 g/kg of this indigestible fraction. However, although linear inclusion of FSG up to 20 g/kg in SF diets increased the fermented fraction, no relevant changes in the fermentation profile were observed. In conclusion, FSG satisfies two essential conditions of the prebiotic effect, showing resistance to in vitro enzymatic digestion and being totally fermented in vitro by caecal bacteria, although in vivo studies will be necessary to determine its prebiotic potential.
This study is supported by the Ministerio de Economia, Industria y Competitividad of the Spanish Government (AGL2017-85162-C2-1R), the Universitat Politecnica de Valencia (Project 20180290; Spain), and the Higher School of Agriculture of Mateur of the Carthage University (Tunisia Republic). The grant for Jihed Zemzmi from the Carthage University is also gratefully acknowledged.
Databáze: OpenAIRE
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