Caveolin-2 in urine as a novel biomarker of renal recovery after cisplatin induced nephrotoxicity in rats
| Autor: | Adriana M. Torres, Romina Paula Bulacio |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Time Factors Caveolin 2 Urine Pharmacology urologic and male genital diseases Toxicology Kidney 03 medical and health sciences 0302 clinical medicine Medicine Animals Rats Wistar Cisplatin business.industry Acute kidney injury General Medicine Recovery of Function Apical membrane Acute Kidney Injury medicine.disease Pathophysiology Disease Models Animal Renal Elimination 030104 developmental biology medicine.anatomical_structure cardiovascular system Biomarker (medicine) business 030217 neurology & neurosurgery Biomarkers medicine.drug |
| Zdroj: | Toxicology letters. 313 |
| ISSN: | 1879-3169 |
| Popis: | Acute kidney injury (AKI) is a heterogeneous clinical syndrome with diverse outcomes. The recovery from AKI has prognostic importance. Little research has been done in order to find biomarkers that can predict recovery from AKI. Cav-2 is one of the main constituents of caveolae and is expressed in kidney. This study analyzed the time course of Cav-2 urinary excretion and renal expression in rats treated with cisplatin. Male Wistar rats were injected with cisplatin (5 mg/kg b.w., i.p.), and the studies were performed after 2, 4 and 14 days. Cav-2 abundance was evaluated in urine, in renal homogenates and in apical membranes by Western blotting. Cav-2 in urine was increased only 14 days after treatment, in the recovery phase of cisplatin-induced AKI. These results show that Cav-2 in urine could be useful as a biomarker of renal recovery, but not as an early biomarker of cisplatin-induced AKI. Cav-2 expression in total renal homogenates was not modified with treatment, but a down-regulation of Cav-2 in apical membranes was observed in treated animals. We hypothesize that Cav-2 internalizes into renal cells from their apical membrane in response to cisplatin, and regulates in this manner different signaling proteins involved in the physiopathology of renal damage. |
| Databáze: | OpenAIRE |
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