Molecular typing of Staphylococcus aureus bloodstream isolates from geriatric patients attending a long-term care Spanish hospital
| Autor: | M. Carmen Mendoza, Fernando Vázquez, M. Rosario Rodicio, Beatriz Guerra, M. Angeles Argudín |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Microbiology (medical) Staphylococcus aureus Micrococcaceae Genotype Health Services for the Aged Bacteremia medicine.disease_cause Polymerase Chain Reaction Microbiology Bacterial Proteins medicine Cluster Analysis Humans DNA Restriction-Modification Enzymes Typing Deoxyribonucleases Type II Site-Specific Aged Aged 80 and over Molecular epidemiology biology SCCmec Surgical wound General Medicine Staphylococcal Infections biology.organism_classification Long-Term Care Hospitals Molecular Typing Spain Wound Infection Multilocus sequence typing Female |
| Zdroj: | Journal of Medical Microbiology. 60:172-179 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.021758-0 |
| Popis: | AllStaphylococcus aureusisolates (n=31) that caused bacteraemia in a Spanish geriatric hospital during 1996–2006 were analysed by a simple, rapid and inexpensive PCR technique based on variations in thehsdS1andhsdS2genes encoding the sequence recognition subunits of the Sau1 restriction–modification (RM) system. An equal number of isolates collected from surgical wounds over the same time period (control group) were similarly characterized. The RM test allocated 75 % of the isolates to the six major clonal complexes (CC1, CC5, CC8, CC22, CC30 and CC45) for which it was developed. However, recognition of minor CCs and precise identification of the circulating clones required more powerful and comprehensive techniques such asspatyping and multilocus sequence typing (MLST), which are more demanding and expensive. The RM test is not intended to replacespaor MLST typing, but may be of use when time, technical and/or financial resources are limited. Overall, nine and seven CCs were detected in bloodstream and wound isolates, respectively. In both groups, CC5 was the most frequent (35.5 % each), followed by CC45 or CC8 (22.6 and 32.3 % of bloodstream and wound isolates, respectively). The frequency of meticillin resistance was lower in bloodstream (16.1 %) than in wound (51.6 %) isolates (P=0.0025). Among the former, sequence type (ST) 5-staphylococcal cassette chromosomemec(SCCmec) II, ST5-SCCmecIV, ST45-SCCmecIV and ST125-SCCmecIV (now dominant in Spanish hospitals) clones were found. Among the wound isolates, nine meticillin-resistant clones were represented, with three of them (ST125-SCCmecIII, ST125-SCCmecV and ST14-SCCmecV) being newly described. |
| Databáze: | OpenAIRE |
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