Characterization of adiposity and metabolism in Lmna-deficient mice
| Autor: | Dedra A. Cutler, Marc L. Reitman, Bernice Marcus-Samuels, Colin L. Stewart, Teresa Sullivan |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Blood Glucose
Male congenital hereditary and neonatal diseases and abnormalities medicine.medical_specialty Lipodystrophy Biophysics Adipose tissue Biology Biochemistry Muscular Dystrophies LMNA Eating Mice Insulin resistance Sex Factors Internal medicine medicine Animals Insulin Tissue Distribution RNA Messenger Muscular dystrophy Muscle Skeletal Molecular Biology Triglycerides Mice Knockout integumentary system nutritional and metabolic diseases Autosomal dominant trait Nuclear Proteins Cell Biology medicine.disease Familial partial lipodystrophy Lamins Disease Models Animal Endocrinology Phenotype Adipose Tissue embryonic structures Body region Female |
| Zdroj: | Biochemical and biophysical research communications. 291(3) |
| ISSN: | 0006-291X |
| Popis: | Dunnigan's Familial Partial Lipodystrophy (FPLD) is an autosomal dominant disease characterized by regional fat loss and insulin resistance. FPLD is caused by mutations in the LMNA gene, which encodes intermediate filaments of the nuclear lamina. Different LMNA mutations cause Emery-Dreifuss muscular dystrophy and/or a dilated cardiomyopathy. It is not known how LMNA mutations cause any of the disease phenotypes. Here we measure physical and metabolic characteristics of Lmna-/- and +/- mice to determine their usefulness as models for FPLD. Lmna-/- mice, which die prematurely of muscular dystrophy, have little fat, but do not show the insulin resistance characteristic of FPLD. Lmna+/- mice, despite treatment with a high fat diet, do not have decreased fat stores or metabolic features of FPLD. We also show, in mice, that Lmna transcripts are expressed at high levels in muscle and adipose tissue, but do not vary by body region or sex. In conclusion, Lmna+/- and -/- mice do not mimic Dunnigan's FPLD, and differential expression of lamins A and C does not appear to contribute to sex- or tissue-specific LMNA phenotypes. |
| Databáze: | OpenAIRE |
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