Novel antimicrobial coatings based on polylactide for plastic biliary stents to prevent post-endoscopic retrograde cholangiography cholangitis
| Autor: | Sonja Lanzinger, Heinrich M. L. Mühlhofer, Rainer Burgkart, J. Tübel, Andreas Weber, Jochen Schneider, Nikolina Lipovcic, Roland M. Schmid, Susanne Feihl, Andreas Obermeier, Philipp Stolte, Silvia Würstle |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) medicine.medical_specialty Cholangitis Polyesters medicine.medical_treatment 030106 microbiology Microbial Sensitivity Tests Gastroenterology Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Humans Pharmacology (medical) 030212 general & internal medicine Agar diffusion test Candida albicans Cholangiopancreatography Endoscopic Retrograde Pharmacology biology Stent Drug-Eluting Stents biology.organism_classification Antimicrobial Anti-Bacterial Agents Triclosan Drug Liberation Infectious Diseases ROC Curve chemistry Toxicity Female Drug carrier Plastics |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 74:1911-1920 |
| ISSN: | 1460-2091 0305-7453 |
| Popis: | Background Cholangitis is a common complication after endoscopic retrograde cholangiography (ERC). Objectives To evaluate antimicrobial coatings for biliary plastic stents in relation to efficacy against biliary pathogens, drug release and toxicity. Methods Biliary plastic stents were prepared by coating using a polylactide drug carrier. Stent coatings contained 4% (w/w) drug content of Resomer-octenidine (RO), Resomer-octenidine and citrate (ROC), Resomer-triclosan (RT) or Resomer-gentamicin (RG). Drug-release kinetics, antimicrobial efficacies of coated biliary stents against biliary pathogens and biocompatibilities were tested. Antimicrobial efficacy measurements included MIC testing, zone of inhibition (ZOI) assays and log reduction in bacterial suspensions. Results Continuous drug release was observed in all antimicrobial stent coatings for at least 168 h with an initial peak within the first 24 h. RT-, ROC- and RG-coated stents resulted in the following log reductions in suspensions: Escherichia coli (-0.3, -7.4 and -6.6, respectively); Enterococcus faecalis (-0.05, -6.3 and -3.9, respectively); and Candida albicans (-0.04, -1.5 and -0.2, respectively). ROC had the highest log reduction in suspension and the most favourable time course of ZOI (≥2 mm, over 72 h) against all tested pathogens. Although RT coatings showed the lowest MICs, they had the lowest ZOIs after 24 h. Concerning RO, acceptable biocompatibility could only be reached by adding a citrate component. RG showed the largest ZOI after 24 h against E. coli (19.3 mm) and E. faecalis (5.1 mm), whereas the ZOI was lower against C. albicans (1.3 mm) compared with ROC (3.7 mm). Conclusions ROC corresponds most closely to the requirements of an ideal antimicrobial stent coating to prevent post-ERC cholangitis, showing the highest log reduction in pathogen counts, the most favourable time course of ZOI and high biocompatibility. |
| Databáze: | OpenAIRE |
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