The role of integrins in Drosophila egg chamber morphogenesis
| Autor: | Holly E. Lovegrove, Daniel St Johnston, Daniel T. Bergstralh |
|---|---|
| Přispěvatelé: | Bergstralh, Daniel [0000-0003-1689-3715], St Johnston, Daniel [0000-0001-5582-3301], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Integrins
Integrin beta Chains Cell Integrin Morphogenesis Mutant clones Germline Basement Membrane 03 medical and health sciences 0302 clinical medicine Cell polarity medicine Animals Drosophila Proteins Molecular Biology 030304 developmental biology Ovum Basement membrane 0303 health sciences Polarity (international relations) biology Polarity Chemistry Cell Polarity Follicle cells Epithelium 3. Good health Cell biology medicine.anatomical_structure Drosophila melanogaster biology.protein Pre-follicle cells Female Carrier Proteins 030217 neurology & neurosurgery Research Article Developmental Biology |
| Zdroj: | Development (Cambridge, England) |
| DOI: | 10.1101/706069 |
| Popis: | The Drosophila egg chamber comprises a germline cyst surrounded by a tightly organised epithelial monolayer, the follicular epithelium (FE). Loss of integrin function from the FE disrupts epithelial organisation at egg chamber termini, but the cause of this phenotype remains unclear. Here, we show that the β-integrin Myospheroid (Mys) is only required during early oogenesis when the pre-follicle cells form the FE. Mutation of mys disrupts both the formation of a monolayered epithelium at egg chamber termini and the morphogenesis of the stalk between adjacent egg chambers, which develops through the intercalation of two rows of cells into a single-cell-wide stalk. Secondary epithelia, like the FE, have been proposed to require adhesion to the basement membrane to polarise. However, Mys is not required for pre-follicle cell polarisation, as both follicle and stalk cells localise polarity factors correctly, despite being mispositioned. Instead, loss of integrins causes pre-follicle cells to constrict basally, detach from the basement membrane and become internalised. Thus, integrin function is dispensable for pre-follicle cell polarity but is required to maintain cellular organisation and cell shape during morphogenesis. Summary: Drosophila integrin mutants exhibit follicle cell multi-layering because mutant pre-follicle cells detach from the basement membrane and become internalised, not because of a loss of polarity or later defects. |
| Databáze: | OpenAIRE |
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