Ribosomal DNA sequences discriminate among toxic and non-toxic Pseudonitzschia species
| Autor: | Greta A. Fryxell, Powers Da, Christopher A. Scholin, Villac Mc, Krupp Jm, Kurt R. Buck, Francisco P. Chavez |
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| Rok vydání: | 1994 |
| Předmět: |
Base pair
Molecular Sequence Data Biology Toxicology DNA Ribosomal Polymerase Chain Reaction law.invention chemistry.chemical_compound Species Specificity law Botany Ribosomal DNA Gene Polymerase chain reaction Genetics Diatoms Kainic Acid Phylogenetic tree Base Sequence Domoic acid Ribosomal RNA chemistry RNA Ribosomal Neuromuscular Depolarizing Agents Phytoplankton Microscopy Electron Scanning Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Natural toxins. 2(4) |
| ISSN: | 1056-9014 |
| Popis: | Cultured isolates of Pseudonitzschia australis Frenguelli, P. delicatissima (Cleve) Heiden, P. americana (Hasle) Fryxell, P. pungens (Grunow) Hasle, and P. pungens f. multiseries (Hasle) Hasle from Monterey Bay, California, were compared on the basis of their large-subunit ribosomal RNA gene (LsrDNA). Pseudonitzschia australis, P. pungens f. multiseries, and P. delicatissima were previously shown to produce the neurotoxin domoic acid; the remaining isolates are considered non-toxic. For each isolate approximately 800 base pairs of LsrDNA, encompassing both evolutionarily conserved and evolutionarily variable regions of the molecule, were amplified using the polymerase chain reaction (PCR) and sequenced. Phylogenetic trees generated by parsimony analysis of aligned sequences afford a preliminary view of the organisms genetic relationships. Species defined by morphological criteria are also distinguishable by LsrDNA sequence. Organisms known or suspected to produce domoic acid cluster at different termini on the phylogenetic tree. Two genetically distinct strains of P. australis and P. pungens were identified. Development of a restriction fragment length polymorphism (RFLP) assay of the LsrDNA is described. The RFLP assay discriminates each species, including distinguished strains of P. australis and P. pungens. The restriction test provides a rapid and convenient method for screening isolates' LsrDNA, facilitating further tests of the apparent positive correlation between Pseudonitzschia species' ribosomal gene signatures, morphology, and capacity to produce domoic acid. copy; 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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