High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine
| Autor: | Fabien Ribes, Patrick Masson, Oksana Lockridge, Florian Nachon, Marie Thérèse Froment, Cedric Matthews, Cynthia F. Bartels |
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| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Stereochemistry Butyrylthiocholine Hydrogen-Ion Concentration Biochemistry Acetylcholinesterase Serine chemistry.chemical_compound Structure-Activity Relationship Enzyme chemistry Amino Acid Substitution Butyrylcholinesterase Catalytic triad Humans Enzyme kinetics Amino Acids Histidine |
| Zdroj: | European journal of biochemistry. 270(2) |
| ISSN: | 0014-2956 |
| Popis: | Butyrylcholinesterase is a serine esterase, closely related to acetylcholinesterase. Both enzymes employ a catalytic triad mechanism for catalysis, similar to that used by serine proteases such as alpha-chymotrypsin. Enzymes of this type are generally considered to be inactive at pH values below 5, because the histidine member of the catalytic triad becomes protonated. We have found that butyrylcholinesterase retains activity at pHor= 5, under conditions of excess substrate activation. This low-pH activity appears with wild-type butyrylcholinesterase as well as with all mutants we examined: A328G, A328I, A328F, A328Y, A328W, E197Q, L286W, V288W and Y332A (residue A328 is at the bottom of the active-site gorge, near the pi-cation-binding site; E197 is next to the active-site serine S198; L286 and V288 form the acyl-binding pocket; and Y332 is a component of the peripheral anionic site). For example, the kcat value at pH 5.0 for activity in the presence of excess substrate was 32900 +/- 4400 min(-1) for wild-type, 55200 +/- 1600 min(-1) for A328F, and 28 700 +/- 700 min(-1) for A328W. This activity is titratable, with pKa values of 6.0-6.6, suggesting that the catalytic histidine is protonated at pH 5. The existence of activity when the catalytic histidine is protonated indicates that the catalytic-triad mechanism of butyrylcholinesterase does not operate for catalysis at low pH. The mechanism explaining the catalytic behaviour of butyrylcholinesterase at low pH in the presence of excess substrate remains to be elucidated. |
| Databáze: | OpenAIRE |
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