Human Monoclonal Antibodies Derived From Memory B Cells Following Live Attenuated Dengue Virus Vaccination or Natural Infection Exhibit Similar Characteristics
Autor: | Ruklanthi de Alwis, Aravinda M. de Silva, James E. Crowe, Nurgun Kose, Scott A. Smith, Stephen S. Whitehead, Anna P. Durbin |
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Rok vydání: | 2013 |
Předmět: |
Herpesvirus 4
Human medicine.drug_class Dengue Vaccines Cross Reactions Dengue virus Antibodies Viral Vaccines Attenuated Monoclonal antibody medicine.disease_cause California Cell Line Dengue fever Dengue Major Articles and Brief Reports Antigen Neutralization Tests North Carolina medicine Humans Immunology and Allergy Antibody-dependent enhancement Antigens Viral Dengue vaccine B-Lymphocytes Hybridomas biology Vaccination Antibodies Monoclonal Dengue Virus medicine.disease Antibodies Neutralizing Antibody-Dependent Enhancement Virology Recombinant Proteins Infectious Diseases Immunology biology.protein Antibody |
Zdroj: | The Journal of Infectious Diseases. 207:1898-1908 |
ISSN: | 1537-6613 0022-1899 |
Popis: | The immunopathogenesis of severe dengue is poorly understood, but there is concern that induction of cross-reactive nonneutralizing antibodies by infection or vaccination may increase the likelihood of severe disease during a subsequent infection. We generated a total of 63 new human monoclonal antibodies to compare the B-cell response of subjects who received the National Institutes of Health live attenuated dengue vaccine rDEN1Δ30 to that of subjects following symptomatic primary infection with DENV1. Both infection and vaccination induced serum neutralizing antibodies and DENV1-reactive peripheral blood B cells, but the magnitude of induction was lower in vaccinated individuals. Serotype cross-reactive weakly neutralizing antibodies dominated the response in both vaccinated and naturally infected subjects. Antigen specificities were very similar, with a slightly greater percentage of antibodies targeting E protein domain I/II than domain III. These data shed light on the similarity of human B-cell response to live attenuated DENV vaccine or natural infection. |
Databáze: | OpenAIRE |
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