In situ generation of iminodiacetic acid groups on nanoporous alumina for the reversible immobilization of enzymes and other biomolecules
| Autor: | Marcus Kjellander, Lennart Berglin, Gunnar Johansson |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Iminodiacetic acid
Metal ions in aqueous solution Kinetics Bioengineering Applied Microbiology and Biotechnology chemistry.chemical_compound Nanopores Lactate dehydrogenase Monolayer Enzyme Stability Aluminum Oxide Animals chemistry.chemical_classification Chromatography L-Lactate Dehydrogenase Nanoporous Biomolecule Imino Acids Muscles General Medicine Enzymes Immobilized Membrane chemistry Chickens Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology letters. 36(9) |
| ISSN: | 1573-6776 |
| Popis: | Nanoporous alumina membranes were silanized with aminopropylsilane and iminodiacetic acid (IDA) groups were generated in situ by reaction with iodoacetate. The membranes were mounted in standard filter holders, connected to a HPLC system and saturated with selected metal ions. Cu(II) allowed the capture of chicken muscle lactate dehydrogenase with such stability, repeatability and reproducibility that Michaelis–Menten kinetics could be studied. The IDA surface was stable for months and could be depleted and regenerated with metal ions multiple times without appreciable loss of capacity. The binding of lactate dehydrogenase influenced the backpressure to the extent that could be expected for a monolayer according to Poiseuilles law. |
| Databáze: | OpenAIRE |
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