Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018

Autor: Michelle Sniatynski, Joseph E. Rubin, Rachel Courtice
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Gram-negative bacteria
040301 veterinary sciences
Veterinary medicine
Urine
Standard Article
Microbial Sensitivity Tests
medicine.disease_cause
Microbiology
0403 veterinary science
03 medical and health sciences
Antibiotic resistance
Dogs
Ampicillin
Drug Resistance
Multiple
Bacterial
SF600-1100
Escherichia coli
Medicine
Nephrology/Urology
Animals
bacterial
Dog Diseases
Escherichia coli Infections
0303 health sciences
General Veterinary
biology
030306 microbiology
business.industry
microbiology
multidrug resistant
04 agricultural and veterinary sciences
gram‐negative bacteria
biology.organism_classification
Antimicrobial
bacterial infections and mycoses
Standard Articles
Saskatchewan
3. Good health
Anti-Bacterial Agents
Multiple drug resistance
Urinary Tract Infections
Multilocus sequence typing
renal
SMALL ANIMAL
business
medicine.drug
Multilocus Sequence Typing
Plasmids
Zdroj: Journal of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine, Vol 35, Iss 3, Pp 1389-1396 (2021)
ISSN: 1939-1676
0891-6640
Popis: Background Urinary tract infections (UTIs) are common in dogs and can be caused by multidrug‐resistant Escherichia coli (E coli). Objective To describe the frequency and mechanisms of antimicrobial resistance (AMR) among E coli causing UTIs in dogs in Western Canada during a 4‐year surveillance period. Animals Urine from 516 dogs. Methods From November 2014 to 2018, 516 nonduplicate E coli isolates from the urine of dogs were collected from a diagnostic laboratory. Susceptibility testing was determined for a panel of 14 antimicrobials belonging to 7 drug classes. Resistant isolates were screened for the presence of extended‐spectrum beta‐lactamases (ESBLs), AmpC β‐lactamases, and plasmid‐mediated quinolone resistance (PMQR) genes. Epidemiological relationships were assessed by MLST. Results 80.2% (414/516) of isolates were susceptible to all antimicrobials tested. There was no significant increase in the proportion of isolates resistant to any of the tested antimicrobials during the study period. Resistance to ampicillin was the most common (14.9%, 77/516). Overall, 12 isolates had bla CMY‐2‐type AmpC β‐lactamases, and 7 produced CTX‐M‐type ESBLs. A single isolate had the aac(6′)‐Ib‐cr PMQR gene. The qnr and qepA determinants were not detected. A single isolate belonging to the pandemic lineage ST131 was identified. Conclusion Escherichia coli isolated from the urine of dogs in our region remain susceptible to first‐line therapies, though resistance, particularly to the aminopenicillins, warrants monitoring. This is the first description of E coli ST131 from a companion animal in Canada.
Databáze: OpenAIRE
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