Covalent binding of hyper-activated Rhizomucor miehei lipase (RML) on hetero-functionalized siliceous supports
| Autor: | Maryam Yousefi, Ali Ramazani, Behrouz Sabour, Maryam Ashjari, Maryam Garmroodi, Mehdi Mohammadi, Javad Mohammadi |
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| Rok vydání: | 2016 |
| Předmět: |
02 engineering and technology
01 natural sciences Biochemistry Hydrophobic effect chemistry.chemical_compound Adsorption Structural Biology Organic chemistry Lipase Rhizomucor Molecular Biology biology 010405 organic chemistry Chemistry Silica gel Chemical modification General Medicine Silanes Enzymes Immobilized Silicon Dioxide 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Enzyme Activation Covalent bond biology.protein Surface modification 0210 nano-technology Mesoporous material Hydrophobic and Hydrophilic Interactions Protein Binding |
| Zdroj: | International Journal of Biological Macromolecules. 86:208-215 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2016.01.076 |
| Popis: | Physical adsorption onto hydrophobic supports has proven to be an effective way to improve the activity of lipases. Covalent binding, on the other hand, enhances the active lifetime of the immobilized biocatalysts. To combine the benefits of adsorption and covalent binding, immobilization of RML on new hetero-functional supports are reported. For this, chemical modification of silica and silica mesoporous nanoparticles was performed by the simultaneous use of two coupling linkers; Octyltriethoxysilane (OTES) for hydrophobic interaction and glycidoxypropyltrimethoxylsilane (GPTMS) for covalent linkage of RML. Altering the GPTMS/OTES ratio makes possible to have different amount of octyl and epoxy groups on the supports. The results showed that immobilization of RML on octyl-functionalized supports produces specific activity almost 1.5-2 folds greater than the specific activity of the free enzyme. The observed hyper-activation decreased with increasing epoxy groups on the supports confirming the enhancement of covalent nature of the attachment. Leaching experiment was also confirmed positive effect of the presence of epoxy groups on the supports. Regarding the specific activity of the immobilized preparations and desorption percentages of RML from each support, the most suitable carrier obtains from the functionalization of the supports in presence of GPTMS and OTES in the ratio of 1:1. |
| Databáze: | OpenAIRE |
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