The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice
| Autor: | Nikhil P. Tewari, Conner R. Valuch, Seungyup Sun, Olatundun D. Awosanya, Anthony J. Perugini, Donghui Zhou, Melissa A. Kacena, Rachel J. Blosser, Caio de Andrade Staut, Rohit U. Nagaraj, Ushashi C. Dadwal, Sarah Lyn Mostardo, Fazal Ur Rehman Bhatti, Stephen K. Mendenhall, Jiliang Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Aging QH301-705.5 Angiogenesis Neovascularization Physiologic Bone Marrow Cells Bone healing bone Heterocyclic Compounds 4 or More Rings Catalysis Article Inorganic Chemistry Andrology angiogenesis SRT1720 Sirtuin 1 Cell Movement medicine Angiopoietin-1 Animals Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Lung Spectroscopy lungs Cell Proliferation Vascular Endothelial Growth Factor Receptor-1 biology business.industry Activator (genetics) Organic Chemistry Endothelial Cells General Medicine Computer Science Applications Mice Inbred C57BL Chemistry medicine.anatomical_structure Impaired bone healing Gene Expression Regulation biology.protein cardiovascular system Female Bone marrow business |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 20 International Journal of Molecular Sciences, Vol 22, Iss 11097, p 11097 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Angiogenesis is critical for successful fracture healing. Age-related alterations in endothelial cells (ECs) may cause impaired bone healing. Therefore, examining therapeutic treatments to improve angiogenesis in aging may enhance bone healing. Sirtuin 1 (SIRT1) is highly expressed in ECs and its activation is known to counteract aging. Here, we examined the effects of SRT1720 treatment (SIRT1 activator) on the growth and function of bone marrow and lung ECs (BMECs and LECs, respectively), derived from young (3–4 month) and old (20–24 month) mice. While aging did not alter EC proliferation, treatment with SRT1720 significantly increased proliferation of all LECs. However, SRT1720 only increased proliferation of old female BMECs. Vessel-like tube assays showed similar vessel-like structures between young and old LECs and BMECs from both male and female mice. SRT1720 significantly improved vessel-like structures in all LECs. No age, sex, or treatment differences were found in migration related parameters of LECs. In males, old BMECs had greater migration rates than young BMECs, whereas in females, old BMECs had lower migration rates than young BMECs. Collectively, our data suggest that treatment with SRT1720 appears to enhance the angiogenic potential of LECs irrespective of age or sex. However, its role in BMECs is sex- and age-dependent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|