Fear conditioning in mouse lines genetically selected for binge-like ethanol drinking
| Autor: | Pamela Metten, K. Matthew Lattal, John C. Crabbe, Jason P. Schlumbohm, Amanda M. Barkley-Levenson, Wyatt R. Hack |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Health (social science) Alcohol Drinking media_common.quotation_subject Physiology Binge drinking Context (language use) Toxicology Biochemistry Article Binge Drinking Mice Random Allocation 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Species Specificity Conditioning Psychological medicine Animals Circadian rhythm Fear conditioning Psychiatry media_common Addiction Fear General Medicine Extinction (psychology) medicine.disease Comorbidity 030227 psychiatry Freezing behavior Neurology Psychology 030217 neurology & neurosurgery |
| Zdroj: | Alcohol. 52:25-32 |
| ISSN: | 0741-8329 |
| DOI: | 10.1016/j.alcohol.2016.01.004 |
| Popis: | The comorbidity of substance- and alcohol-use disorders (AUD) with other psychiatric conditions, especially those related to stress such as post-traumatic stress disorder (PTSD), is well-established. Binge-like intoxication is thought to be a crucial stage in the development of the chronic relapsing nature of the addictions, and self-medication through binge-like drinking is commonly seen in PTSD patients. We have selectively bred two separate High Drinking in the Dark (HDID-1 and HDID-2) mouse lines to reach high blood ethanol concentrations (BECs) after a 4-h period of access to 20% ethanol starting shortly after the onset of circadian dark. As an initial step toward the eventual goal of employing binge-prone HDID mice to study PTSD-like behavior including alcohol binge drinking, we sought first to determine their ability to acquire conditioned fear. We asked whether these mice acquired, generalized, or extinguished conditioned freezing to a greater or lesser extent than unselected control HS/Npt mice. In two experiments, we trained groups of 16 adult male mice in a standard conditioned fear protocol. Mice were tested for context-elicited freezing, and then, in a novel context, for cue-induced freezing. After extinction tests, renewal of conditioned fear was tested in the original context. Mice of all three genotypes showed typical fear responding. Context paired with shock elicited freezing behavior in a control experiment, but cue unpaired with shock did not. These studies indicate that fear learning per se does not appear to be influenced by genes causing predisposition to binge drinking, suggesting distinct neural mechanisms. However, HDID mice are shown to be a suitable model for studying the role of conditioned fear specifically in binge-like drinking. |
| Databáze: | OpenAIRE |
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