Custodiol-N Is Superior to Custodiol® Solution in Experimental Rat Uterus Preservation
| Autor: | Vygante Maskoliunaite, Diana Ramasauskaite, Philipp Stiegler, Mindaugas Kvietkauskas, Bettina Leber, Peter Schemmer, Viktorija Zitkute, Kęstutis Strupas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Necrosis
preservation Ischemia Uterus medicine.disease_cause Catalysis static cold storage Inorganic Chemistry Andrology lcsh:Chemistry 03 medical and health sciences 0302 clinical medicine Uterus transplantation medicine Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy ischemia reperfusion injury 030219 obstetrics & reproductive medicine biology business.industry Organic Chemistry Histology General Medicine medicine.disease Computer Science Applications medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 030220 oncology & carcinogenesis Myeloperoxidase biology.protein medicine.symptom business uterus transplantation infertility Reperfusion injury Oxidative stress |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 8015, p 8015 (2020) International Journal of Molecular Sciences Volume 21 Issue 21 International journal of molecular sciences, Basel : MDPI, 2020, vol. 21, iss. 21, art. no. 8015, p. [1-10] |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Uterus transplantation (UTx) is the first and only available treatment for women with absolute uterine factor infertility. However, clinical application is limited by the lack of organs, ischemia/reperfusion injury, as well as immunosuppression after UTx. Several different preservation solutions are used in experimental and clinical UTx, including Custodiol® solution. Recently, the novel Custodiol-N solution was developed with superior results in organ preservation. However, the solution was not tested yet in UTx. Therefore, the aims of this study were to evaluate the effect of Custodiol-N in uterus prolonged cold preservation time (8 and 24 h), compared to Custodiol® solution. Uterus tissue samples were obtained from adult Sprague Dawley rats (n = 10/group). Cold ischemic injury was estimated by histology, including immunohistochemistry, and biochemical tissue analyses. After 8 h of cold ischemia, higher percentage of tissue edema, necrosis signs and myeloperoxidase expression, as well as lower superoxide dismutase activity were found in Custodiol® compared to Custodiol-N (p < 0.05). These differences were more pronounced after 24 h of cold preservation time (p < 0.05). This study demonstrated that Custodiol-N protects uterus grafts from cold ischemic injury better than standard Custodiol® most likely via inhibition of oxidative stress and tissue edema. It seems that iron chelators in the composition of Custodiol-N play an important protective role against cold ischemia. |
| Databáze: | OpenAIRE |
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