Octopamine stabilizes conduction reliability of an unmyelinated axon during hypoxic stress
| Autor: | Kevin P. Cross, M. K. J. Sproule, Tomas G. A. Money, R. M. Robertson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nervous system Male Patch-Clamp Techniques Time Factors Interneuron Physiology Movement Neural Conduction Action Potentials Grasshoppers Nerve conduction velocity Functional Laterality 03 medical and health sciences 0302 clinical medicine Cellular and Molecular Properties of Neurons Neuropil medicine Animals Patch clamp Axon Enzyme Inhibitors Hypoxia Sodium Azide Octopamine Hyperbaric Oxygenation Nerve Fibers Unmyelinated Thoracic Nerves Chemistry General Neuroscience 030104 developmental biology medicine.anatomical_structure nervous system Neuron Neuroscience Adrenergic alpha-Agonists 030217 neurology & neurosurgery Photic Stimulation |
| Popis: | Mechanisms that could mitigate the effects of hypoxia on neuronal signaling are incompletely understood. We show that axonal performance of a locust visual interneuron varied depending on oxygen availability. To induce hypoxia, tracheae supplying the thoracic nervous system were surgically lesioned and action potentials in the axon of the descending contralateral movement detector (DCMD) neuron passing through this region were monitored extracellularly. The conduction velocity and fidelity of action potentials decreased throughout a 45-min experiment in hypoxic preparations, whereas conduction reliability remained constant when the tracheae were left intact. The reduction in conduction velocity was exacerbated for action potentials firing at high instantaneous frequencies. Bath application of octopamine mitigated the loss of conduction velocity and fidelity. Action potential conduction was more vulnerable in portions of the axon passing through the mesothoracic ganglion than in the connectives between ganglia, indicating that hypoxic modulation of the extracellular environment of the neuropil has an important role to play. In intact locusts, octopamine and its antagonist, epinastine, had effects on the entry to, and recovery from, anoxic coma consistent with octopamine increasing overall neural performance during hypoxia. These effects could have functional relevance for the animal during periods of environmental or activity-induced hypoxia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|