Ligand-Peroxidase Conjugates for Quantification of Receptor-Mediated Transport into Cells
| Autor: | Amandio Vieira, Jie Chen |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cell type
Chemistry Ligand Cellular differentiation media_common.quotation_subject Organic Chemistry Transferrin Biotin Biological Transport General Medicine Receptor-mediated endocytosis Ligands Computer Science Applications Biochemistry In vivo Cell Line Tumor Biotinylation Drug Discovery Humans Streptavidin Internalization Peroxidase media_common Conjugate |
| Zdroj: | Combinatorial Chemistry & High Throughput Screening. 16:320-323 |
| ISSN: | 1386-2073 |
| DOI: | 10.2174/1386207311316040005 |
| Popis: | Receptor-mediated cellular uptake of physiological regulators such as nutritional and hormonal factors represents a transport event with important consequences for cell differentiation, death, or proliferation. Although internalization pathways are important points of regulation, they have not been extensively explored as pharmacological targets in most cell types. An experimental strategy based on ligand-enzyme conjugates is presented in this report that may facilitate high-throughput screening for potent chemical modulators of the transport events. The method was tested on a human epidermoid cell line using a streptavidin-peroxidase conjugate, and human holo-transferrin as the model ligand, in a biotin-ligand conjugate. The proposed screening method is rapid, can be performed using multi-well plates, and involves small assay volumes. The modular nature of the ligand complex makes this method adaptable to the use of other biotinylated ligands, and the use of avidins conjugated to other enzymes. As is discussed, the method may also be applicable to other in vitro and in vivo transport assays. |
| Databáze: | OpenAIRE |
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