Tooth replacement and putative odontogenic stem cell niches in pharyngeal dentition of medaka (Oryzias latipes)
| Autor: | Makoto J. Tabata, Hiroshi Mitani, Kazunori Higuchi, Otto Baba, Yoshiro Takano, Dawud Abduweli |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Mesoderm
Oryzias In situ hybridization Biology stomatognathic system SOX2 Structural Biology medicine Animals Regeneration Radiology Nuclear Medicine and imaging Stem Cell Niche Instrumentation Staining and Labeling Dentition SOXB1 Transcription Factors Stem Cells Regeneration (biology) fungi Tooth Germ Anatomy Pharyngeal teeth biology.organism_classification stomatognathic diseases medicine.anatomical_structure Odontogenesis Pharynx Stem cell Tooth |
| Zdroj: | Microscopy. 63:141-153 |
| ISSN: | 2050-5701 2050-5698 |
| Popis: | The small-sized teleost fish medaka, Oryzias latipes, has as many as 1000 pharyngeal teeth undergoing continuous replacement. In this study, we sought to identify the tooth-forming units and determine its replacement cycles, and further localize odontogenic stem cell niches in the pharyngeal dentition of medaka to gain insights into the mechanisms whereby continuous tooth replacement is maintained. Three-dimensional reconstruction of pharyngeal epithelium and sequential fluorochrome labeling of pharyngeal bones and teeth indicated that the individual functional teeth and their successional teeth were organized in families, each comprising up to five generations of teeth and successional tooth germs, and that the replacement cycle of functional teeth was approximately 4 weeks. BrdU label/chase experiments confirmed the existence of clusters of label-retaining epithelial cells at the posterior end of each tooth family where the expression of pluripotency marker Sox2 was confirmed by in situ hybridization. Label-retaining cells were also identified in the mesoderm immediately adjacent to the posterior end of each tooth family. These data suggest the importance of existence of slow-cycling dental epithelial cells and Sox2 expressions at the posterior end of each tooth family to maintain continuous tooth formation and replacement in the pharyngeal dentition of medaka. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|