Pigment epithelium-derived factor exerts opposite effects on endothelial cells of different phenotypes

Autor: Jean Plouët, Joyce Tombran-Tink, Martine Michele Maitre-Boube, Helen Hutchings
Rok vydání: 2002
Předmět:
Vascular Endothelial Growth Factor A
Time Factors
Endothelium
MAP Kinase Signaling System
Blotting
Western
Biophysics
Endothelial Growth Factors
Fibroblast growth factor
Biochemistry
Retina
chemistry.chemical_compound
PEDF
medicine
Animals
Humans
Nerve Growth Factors
Phosphorylation
Eye Proteins
Hypoxia
Molecular Biology
Serpins
Mitogen-Activated Protein Kinase 1
Lymphokines
Mitogen-Activated Protein Kinase 3
Dose-Response Relationship
Drug
Neovascularization
Pathologic
Reverse Transcriptase Polymerase Chain Reaction
Vascular Endothelial Growth Factors
Proteins
Cell Biology
Precipitin Tests
Cell biology
Vascular endothelial growth factor
Vascular endothelial growth factor B
Vascular endothelial growth factor A
Phenotype
medicine.anatomical_structure
chemistry
Vascular endothelial growth factor C
Immunology
Cattle
Mitogen-Activated Protein Kinases
Cell Division
Zdroj: Biochemical and Biophysical Research Communications. 294:764-769
ISSN: 0006-291X
DOI: 10.1016/s0006-291x(02)00548-x
Popis: The anti-angiogenic activity of pigment epithelium-derived factor (PEDF) has recently been discovered on the basis of its inhibition of ischemia-induced retinal neovascularization in an animal model of retinopathy of the premature. Moreover PEDF inhibits the migration and proliferation of various endothelial cells maintained in culture with FGF(2). Since vascular endothelial growth factor (VEGF) is the main angiogenic factor expressed in hypervascularized retinas, we investigated the functions of PEDF on retinal endothelial cells whose angiogenic phenotype is controlled or not by long term exposure to VEGF as observed in human pathologies such as diabetic retinopathy. Here, we observed that PEDF exerts opposite effects on endothelial cells depending on their phenotype. We determined that when PEDF inhibits endothelial cell growth, it inhibits VEGF-induced MAPK activation. However, in endothelial cells cultured with VEGF, PEDF has a synergistic action on cell proliferation with VEGF, and this corresponds to increased MAPK activation.
Databáze: OpenAIRE
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