Overexpression of reactive oxygen species modulator 1 is associated with advanced grades of bladder cancer
| Autor: | Hadi Ghasemi, Atefeh Pegah, Shima Khanverdilou, Seyed Habibollah Mousavibahar, Mohammad Amin Amini, Heidar Tayebinia, Aida Alizamir, Ebrahim Azizi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.disease_cause law.invention Mitochondrial Proteins 03 medical and health sciences 0302 clinical medicine law Gene expression Genetics medicine Biomarkers Tumor Humans Tumor marker Molecular Biology Gene Polymerase chain reaction Aged ROMO1 chemistry.chemical_classification Reactive oxygen species modulator 1 Reactive oxygen species Bladder cancer business.industry Membrane Proteins General Medicine medicine.disease Up-Regulation Gene Expression Regulation Neoplastic Oxidative Stress 030104 developmental biology chemistry Urinary Bladder Neoplasms 030220 oncology & carcinogenesis Cancer research Original Article Neoplasm Grading business Reactive Oxygen Species Oxidative stress |
| Zdroj: | Molecular Biology Reports |
| ISSN: | 1573-4978 |
| Popis: | Reactive Oxygen Species Modulator 1 (ROMO1) plays a pivotal role in the regulation of mitochondrial structure integrity, and the production of reactive oxygen species (ROS). Increased ROMO1 expression was reported in various cancer cell lines; however, the possible association between ROMO1 expression and bladder cancer was not well studied. The present study aimed to investigate the rate of ROMO1 expression and the correlation of oxidative stress with the development of bladder cancer. In this study, a total of 35 cancerous and healthy adjacent tissues were examined using quantitative real-time polymerase chain reaction (qRT-PCR) to analyze the gene expression of ROMO1. Also, we evaluated the serum level of ROMO1 and Total Antioxidant Capacity (TAC), as well as Total Oxidant Status (TOS) in patients with bladder cancer along with age- and sex-matched healthy individuals. The ROMO1 gene was significantly higher in cancerous tissues than that of adjacent healthy tissues. Also, the serum levels of ROMO1, TAC, TOS, and Oxidative Stress Index (OSI) were increased in patients with bladder cancer compared with healthy subjects. It can be concluded that the overexpression of the ROMO1 gene is associated with advanced grades of bladder cancer as well as an increase in oxidative stress conditions. Our findings also suggest that the serum level of ROMO1 might be a promising tumor marker for bladder cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink