Isolation of influenza A(H3N2) virus with 'O'→'D' phase variation
| Autor: | S. Ciappi, R. De Santis, Alberta Azzi, C.A. Stein, J.S. Oxford |
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| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
Hemagglutination Influenza A Virus H3N2 Subtype Molecular Sequence Data Immunology Orthomyxoviridae Embryonated Nucleic acid sequence Hemagglutinin Biology biology.organism_classification Antigenic Variation Polymerase Chain Reaction Virology Virus Amino acid chemistry Influenza A virus Humans Amino Acid Sequence Glycoprotein |
| Zdroj: | Research in Virology. 148:427-431 |
| ISSN: | 0923-2516 |
| DOI: | 10.1016/s0923-2516(97)83632-1 |
| Popis: | Summary We report the isolation of two influenza A(H 3 N 2 ) virus strains which were unable, in the first passages in MDCK cell culture, to agglutinate chicken erythrocytes, though reacting with guinea pig and turkey red blood cells. This observation demonstrates that the occurrence of this phenomenon is not exclusive to influenza Al(H 1 hN 1 ) viruses, as previously reported. In order to investigate the molecular basis of this phenomenon, we analysed the nucleotide sequence of the HA-1 region, presumed to be involved in the switch of haemagglutination properties, from the virus present in the original samples and in the corresponding strains isolated and cultivated in MDCK cells and in embryonated eggs. The substitution of amino acid 138 (Ala→Ser) in MDCK cells could be related to the change in haemagglutination characteristics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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