Genomic organization and full-length cDNA sequence of human collagen X
| Autor: | Bjorn R. Olsen, Frank Beier, Wolf Bertling, Klaus von der Mark, Phyllis LuValle, Ernst J Reichenberger |
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| Rok vydání: | 1992 |
| Předmět: |
Evolution
Sequence analysis Molecular Sequence Data Restriction Mapping Biophysics Regulatory Sequences Nucleic Acid Biology Polymerase Chain Reaction Biochemistry Regulatory sequence Exon Rapid amplification of cDNA ends Structural Biology Sequence Homology Nucleic Acid Complementary DNA Genetics Animals Amino Acid Sequence Molecular Biology Genomic organization Genomic Library Base Sequence Nucleic acid sequence Intron Promoter DNA Cell Biology Human cartilage Molecular biology Oligodeoxyribonucleotides Human type X Collagen Nucleotide sequence Chickens |
| Zdroj: | FEBS Letters. 311:305-310 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(92)81126-7 |
| Popis: | We have determined the full-length cDNA sequence of the human alpha 1(X) collagen gene by sequence analysis of a genomic clone ERG [(1991) Dev. Biol. 148, 562-572], and of cDNA fragments generated from a reverse transcribed as alpha 1(X) mRNA by PCR. We defined the promoter region, the transcription initiation site and the full-length 5'-untranslated region. We also report the exon/intron boundaries of the transcript and the complete 3'-untranslated region as well as a 3'-flanking sequence containing two additional polyadenylation signals. The promoter region is homologous to chicken and mouse type X promoters within several highly conserved regions. The genomic organization shows high homologies to chicken and mouse. |
| Databáze: | OpenAIRE |
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