Acute inhibition of monoamine oxidase and ischemic preconditioning in isolated rat hearts: interference with postischemic functional recovery but no effect on infarct size reduction
| Autor: | Andreea Privistirescu, Silvia N Mirica, Lavinia Noveanu, Danina Muntean, Adrian Sturza, Oana Duicu, Valentin Ordodi, Maria D. Dănilă |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Clorgyline Monoamine Oxidase Inhibitors Physiology Monoamine oxidase Ischemia Myocardial Infarction Pharmacology Ventricular Function Left Contractility Physiology (medical) parasitic diseases medicine Animals cardiovascular diseases Cardioprotection Chemistry General Medicine Recovery of Function medicine.disease Pargyline Rats Monoamine neurotransmitter Anesthesia Ischemic Preconditioning Myocardial Ischemic preconditioning Female medicine.drug |
| Zdroj: | Canadian journal of physiology and pharmacology. 93(9) |
| ISSN: | 1205-7541 |
| Popis: | Monoamine oxidases (MAOs) have recently emerged as important mitochondrial sources of oxidative stress in the cardiovascular system. Generation of reactive oxygen species during the brief episodes of ischemic preconditioning (IPC) is responsible for the cardioprotection at reperfusion. The aim of this study was to assess the effects of two MAO inhibitors (clorgyline and pargyline) on the IPC-related protection in isolated rat hearts. Animals subjected to 30 min global ischemia and 120 min reperfusion were assigned to the following groups: (i) Control, no additional intervention; (ii) IPC, 3 cycles of 5 min ischemia and 5 min reperfusion before the index ischemia; (iii) IPC-clorgyline, IPC protocol bracketed for 5 min with clorgyline (50 μmol/L); (iv) IPC-pargyline, IPC protocol bracketed for 5 min with pargyline (0.5 mmol/L). The postischemic functional recovery was assessed by the left ventricular developed pressure (LVDP) and the indices of contractility (+dLVP/dt max) and relaxation (–dLVP/dt max). Infarct size (IS) was quantified by TTC staining. In both genders, IPC significantly improved functional recovery that was further enhanced in the presence of either clorgyline or pargyline. IS reduction was comparable among all the preconditioned groups, regardless of the presence of MAO inhibitors. In isolated rat hearts, acute inhibition of MAOs potentiates the IPC-induced postischemic functional recovery without interfering with the anti-necrotic protection. |
| Databáze: | OpenAIRE |
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