Direct visualization of an antidepressant analog using surface-enhanced Raman scattering in the brain
Autor: | Yukio Ago, Kazuki Bando, Satoshi Kawata, Kazuya Kikuchi, Masayoshi Arai, Kaoru Seiriki, Atsushi Kimishima, Katsumasa Fujita, Kazuo Harada, Masato Tanuma, Hitoshi Hashimoto, Atsushi Kasai, Naoyuki Kotoku, Masafumi Minoshima, Kosuke Higashino |
---|---|
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
In situ Serotonin reuptake inhibitor Neuroimaging Citalopram Spectrum Analysis Raman Multimodal Imaging Silver nanoparticle Mice 03 medical and health sciences symbols.namesake 0302 clinical medicine Fluorescence microscope Animals Chemistry Resolution (electron density) Brain General Medicine 030104 developmental biology Technical Advance Microscopy Fluorescence 030220 oncology & carcinogenesis symbols Biophysics Antidepressive Agents Second-Generation Bioorthogonal chemistry Selective Serotonin Reuptake Inhibitors Raman scattering |
Zdroj: | JCI Insight |
ISSN: | 2379-3708 |
Popis: | Detailed spatial information of low-molecular weight compound distribution, especially in the brain, is crucial to understanding their mechanism of actions. Imaging techniques that can directly visualize drugs in the brain at a high resolution will complement existing tools for drug distribution analysis. Here, we performed surface-enhanced Raman scattering (SERS) imaging using a bioorthogonal alkyne tag to visualize drugs directly in situ at a high resolution. Focusing on the selective serotonin reuptake inhibitor S-citalopram (S-Cit), which possesses a nitrile group, we substituted an alkynyl group into its structure and synthesized alkynylated S-Cit (Alk-S-Cit). The brain transitivity and the serotonin reuptake inhibition of Alk-S-Cit were not significantly different as compared with S-Cit. Alk-S-Cit was visualized in the coronal mouse brain section using SERS imaging with silver nanoparticles. Furthermore, SERS imaging combined with fluorescence microscopy allowed Alk-S-Cit to be visualized in the adjacent neuronal membranes, as well as in the brain vessel and parenchyma. Therefore, our multimodal imaging technique is an effective method for detecting low-molecular weight compounds in their original tissue environment and can potentially offer additional information regarding the precise spatial distribution of such drugs. |
Databáze: | OpenAIRE |
Externí odkaz: |