Membrane fluidization by alcohols inhibits DesK-DesR signalling in Bacillus subtilis

Autor: Larisa E. Cybulski, Małgorzata Jemioła-Rzemińska, Radovan Fišer, Petra Liskova, Ivo Konopásek, Kateřina Vaňousová, Jana Beranová, Kazimierz Strzałka
Rok vydání: 2017
Předmět:
0301 basic medicine
Fatty Acid Desaturases
Membrane Fluidity
Recombinant Fusion Proteins
education
030106 microbiology
Amino Acid Motifs
Biophysics
Fluorescence Polarization
Bacillus subtilis
Biochemistry
alcohols
Cell membrane
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Genes
Reporter
membrane-active compounds
medicine
Membrane fluidity
Phosphorylation
biology
Calorimetry
Differential Scanning
Butanol
membrane fluidity
Cell Membrane
Fatty Acids
Cell Biology
Gene Expression Regulation
Bacterial
biology.organism_classification
Two-component regulatory system
Cold Temperature
cold shock
030104 developmental biology
Membrane
medicine.anatomical_structure
chemistry
two-component system
Benzyl alcohol
Alcohols
Enzyme Induction
Hydrophobic and Hydrophilic Interactions
Protein Kinases
Protein Processing
Post-Translational
Hexanol
Signal Transduction
Zdroj: Biochimica et biophysica acta. Biomembranes. 1860(3)
ISSN: 0005-2736
Popis: After cold shock, the Bacillus subtilis desaturase Des introduces double bonds into the fatty acids of existing membrane phospholipids. The synthesis of Des is regulated exclusively by the two-component system DesK/DesR; DesK serves as a sensor of the state of the membrane and triggers Des synthesis after a decrease in membrane fluidity. The aim of our work is to investigate the biophysical changes in the membrane that are able to affect the DesK signalling state. Using linear alcohols (ethanol, propanol, butanol, hexanol, octanol) and benzyl alcohol, we were able to suppress Des synthesis after a temperature downshift. The changes in the biophysical properties of the membrane caused by alcohol addition were followed using membrane fluorescent probes and differential scanning calorimetry. We found that the membrane fluidization induced by alcohols was reflected in an increased hydration at the lipid-water interface. This is associated with a decrease in DesK activity. The addition of alcohol mimics a temperature increase, which can be measured isothermically by fluorescence anisotropy. The effect of alcohols on the membrane periphery is in line with the concept of the mechanism by which two hydrophilic motifs located at opposite ends of the transmembrane region of DesK, which work as a molecular caliper, sense temperature-dependent variations in membrane properties.
Databáze: OpenAIRE
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