Identification and comparative analysis of hepatitis B virus genotype D/E recombinants in Africa
| Autor: | Adjoa Obo-Akwa, Awewura Kwara, Ernest Kenu, Kwamena W. Sagoe, Timothy N. Archampong, Margaret Lartey, Jason T. Blackard, Ceejay L. Boyce, Lilia Ganova-Raeva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hepatitis B virus Genotype Genome Viral Biology medicine.disease_cause Genome Article 03 medical and health sciences 0302 clinical medicine Virology Genetics Consensus sequence medicine Humans Genetic variability Molecular Biology Phylogeny Recombination Genetic Whole genome sequencing Phylogenetic tree General Medicine Hepatitis B medicine.disease 030104 developmental biology Africa 030211 gastroenterology & hepatology |
| Zdroj: | Virus Genes. 53:538-547 |
| ISSN: | 1572-994X 0920-8569 |
| Popis: | Globally, there are approximately 240 million people chronically infected with hepatitis B virus (HBV) – a major cause of hepatocellular carcinoma. Ten different HBV genotypes (A-J) have been identified with distinct geographic distributions. Novel variants generated by recombination between different HBV genotypes have been documented worldwide and represent an important element of genetic variability with possible clinical implications. Here, the complete genome sequence of an HBV genotype D/E recombinant from Ghana is reported. The full-length sequence was obtained using rolling circle amplification followed by PCR and then sequenced using next generation sequencing (NGS). A consensus sequence was extracted from the NGS data and underwent phylogenetic analysis to determine genotype, as well as the recombination pattern. Subsequently, the sequence was compared to recombinants described previously in Africa. Based on MCMC phylogenetic analysis, SimPlot recombination analyses, and intragroup genetic distance, the isolate 007N full-length genome is unique compared to other reported D/E recombinants in Africa. |
| Databáze: | OpenAIRE |
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