A comparative study between spanish and british sars-cov-2 variants
| Autor: | José Luis López-Campos, Cecilia López Ramírez, Jose A. Jimenez Ruiz |
|---|---|
| Přispěvatelé: | Universidad de Sevilla. Departamento de Medicina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Antigenicity QH301-705.5 In silico Genomics characterization Biology Microbiology Genome Homology (biology) Humans Amino Acid Sequence Biology (General) Molecular Biology Sequence (medicine) Genetics Whole genome sequencing Base Sequence SARS-CoV-2 Computational Biology COVID-19 General Medicine Virus Internalization Ligand (biochemistry) United Kingdom Computational docking Docking (molecular) Spain Spike Glycoprotein Coronavirus Angiotensin-Converting Enzyme 2 Sequence Alignment Protein Binding |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Current Issues in Molecular Biology Volume 43 Issue 3 Pages 140-2047 Current Issues in Molecular Biology, Vol 43, Iss 140, Pp 2036-2047 (2021) |
| Popis: | The study of the interaction between the SARS-CoV-2 spike protein and the angiotensin-converting enzyme 2 (ACE2) receptor is key to understanding binding affinity and stability. In the present report, we sought to investigate the differences between two already sequenced genome variants (Spanish and British) of SARS-CoV-2. Methods: In silico model evaluating the homology, identity and similarity in the genome sequence and the structure and alignment of the predictive spike by computational docking methods. Results: The identity results between the Spanish and British variants of the Spike protein were 28.67%. This close correspondence in the results between the Spanish and British SARS-CoV-2 variants shows that they are very similar (99.99%). The alignment obtained results in four deletions. There were 23 nucleotide substitutions also predicted which could affect the functionality of the proteins produced from this sequence. The interaction between the binding receptor domain from the spike protein and the ACE2 receptor produces some of the mutations found and, therefore, the energy of this ligand varies. However, the estimated antigenicity of the British variant is higher than its Spanish counterpart. Conclusions: Our results indicate that minimal mutations could interfere in the infectivity of the virus due to changes in the fitness between host cell recognition and interaction proteins. In particular, the N501Y substitution, situated in the RBD of the spike of the British variant, might be the reason for its extraordinary infective potential. |
| Databáze: | OpenAIRE |
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