Orexin-A increases the activity of globus pallidus neurons in both normal and parkinsonian rats
Autor: | Hua Chen, Xin-Yi Chen, Wen-Fang Chen, An-Qi Chen, Hong-Xia Liu, Ying Wang, Yu-Ting Yang, Yan Xue, Hong-Yun Liu, Qing Sheng, Lei Chen, Ya-Yan Pang |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Receptor expression Action Potentials Neuropeptide Biology Globus Pallidus 03 medical and health sciences Orexin-A 0302 clinical medicine Orexin Receptors mental disorders Basal ganglia Animals Urea Single-unit recording Naphthyridines Rats Wistar Oxidopamine Postural Balance Neurons Benzoxazoles Orexins musculoskeletal neural and ocular physiology General Neuroscience digestive oral and skin physiology Parkinson Disease Rats nervous system diseases Orexin Electrophysiology 030104 developmental biology Globus pallidus nervous system Haloperidol Orexin Receptor Antagonists Neuroscience 030217 neurology & neurosurgery |
Zdroj: | European Journal of Neuroscience. 44:2247-2257 |
ISSN: | 0953-816X |
DOI: | 10.1111/ejn.13323 |
Popis: | Orexin is a member of neuropeptides which was first identified in the hypothalamus. The globus pallidus is a key structure in the basal ganglia, which is involved in both normal motor function and movement disorders. Morphological studies have shown the expression of both OX1 and OX2 receptors in the globus pallidus. Employing single unit extracellular recordings and behavioural tests, the direct in vivo electrophysiological and behavioural effects of orexin-A in the globus pallidus were studied. Micro-pressure administration of orexin-A significantly increased the spontaneous firing rate of pallidal neurons. Correlation analysis revealed a negative correlation between orexin-A induced excitation and the basal firing rate. Furthermore, application of the specific OX1 receptor antagonist, SB-334867, decreased the firing rate of pallidal neurons, suggesting that endogenous orexinergic systems modulate the firing activity of pallidal neurons. Orexin-A increased the excitability of pallidal neurons through both OX1 and OX2 receptors. In 6-hydroxydopamine parkinsonian rats, orexin-A-induced increase in firing rate of pallidal neurons was stronger than that in normal rats. Immunostaining revealed positive OX1 receptor expression in the globus pallidus of both normal and parkinsonian rats. Finally, postural test showed that unilateral microinjection of orexin-A led to contralateral deflection in the presence of systemic haloperidol administration. Further elevated body swing test revealed that pallidal orexin-A and SB-334867 induced contralateral-biased swing and ipsilateral-biased swing respectively. Based on the electrophysiological and behavioural findings of orexin-A in the globus pallidus, the present findings may provide a rationale for the pathogenesis and treatment of Parkinson's disease. |
Databáze: | OpenAIRE |
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