Pax6 Is a Human Neuroectoderm Cell Fate Determinant
| Autor: | Jing Chen, Gennadiy I. Bondarenko, Jin Li, Jiajie Xi, Su-Chun Zhang, Timothy M. LaVaute, Zhong Wei Du, Melvin Ayala, Thaddeus G. Golos, Ying Yang, Cindy Tzu-Ling Huang, Xiaoqing Zhang, Xue-Jun Li, Matthew T. Pankratz, Ying Jin |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
PAX6 Transcription Factor Cellular differentiation Mice SCID Cell fate determination Biology In Vitro Techniques Models Biological Article Cell Line 03 medical and health sciences Mice 0302 clinical medicine Transcriptional regulation Genetics Animals Humans Paired Box Transcription Factors Eye Proteins Transcription factor Embryonic Stem Cells 030304 developmental biology Homeodomain Proteins 0303 health sciences Gene knockdown Neural Plate Neuroectoderm Teratoma Cell Differentiation Cell Biology Embryonic stem cell Molecular biology STEMCELL eye diseases Repressor Proteins embryonic structures Molecular Medicine PAX6 sense organs 030217 neurology & neurosurgery |
| Zdroj: | Cell Stem Cell. 7(1):90-100 |
| ISSN: | 1934-5909 |
| DOI: | 10.1016/j.stem.2010.04.017 |
| Popis: | The transcriptional regulation of neuroectoderm (NE) specification is unknown. Here we show that Pax6 is uniformly expressed in early NE cells of human fetuses and those differentiated from human embryonic stem cells (hESCs). This is in contrast to the later expression of Pax6 in restricted mouse brain regions. Knockdown of Pax6 blocks NE specification from hESCs. Overexpression of either Pax6a or Pax6b, but not Pax6triangle upPD, triggers hESC differentiation. However, only Pax6a converts hESCs to NE. In contrast, neither loss nor gain of function of Pax6 affects mouse NE specification. Both Pax6a and Pax6b bind to pluripotent gene promoters but only Pax6a binds to NE genes during human NE specification. These findings indicate that Pax6 is a transcriptional determinant of the human NE and suggest that Pax6a and Pax6b coordinate with each other in determining the transition from pluripotency to the NE fate in human by differentially targeting pluripotent and NE genes. |
| Databáze: | OpenAIRE |
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