Demonstration of autoantibody binding to muscarinic acetylcholine receptors in the salivary gland in primary Sjögren's syndrome
| Autor: | Erzsébet Fehér, Lakszlo Kovacs, Ilona Marczinovits, Viktoria Boda, Janos Somos, György M. Nagy, Ibolya Bodnár |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Biopsy Immunology Salivary Glands stomatognathic system Antibody Specificity Muscarinic acetylcholine receptor medicine Humans Immunology and Allergy Microscopy Immunoelectron Receptor Autoantibodies biology Salivary gland Autoantibody Middle Aged Immunohistochemistry Receptors Muscarinic eye diseases Epithelium stomatognathic diseases Sjogren's Syndrome medicine.anatomical_structure biology.protein Female Antibody Acetylcholine medicine.drug |
| Zdroj: | Clinical Immunology. 128:269-276 |
| ISSN: | 1521-6616 |
| DOI: | 10.1016/j.clim.2008.04.001 |
| Popis: | A significant pathogenetic role of antimuscarinic acetylcholine receptor-3 (anti-m3AChR) autoantibodies in primary Sjögren's syndrome (pSS) has been suggested. However, the binding of these antibodies to the receptors in the target tissues has not yet been demonstrated. In this study, the binding characteristics of pSS sera and anti-m3AChR-monospecific sera affinity-purified from pSS patients to labial salivary gland samples from healthy subjects were studied with light- and electron microscopy. Furthermore, the ultrastructural localisation of in vivo deposited antibodies in pSS salivary glands was also investigated. Light microscopic immunohistochemistry revealed the binding of the anti-m3AChR-specific sera to the membrane of acinar cells. Similar reaction end-products were observed in the pSS salivary gland epithelial cell membranes. With electron microscopy, the autoantibody binding was observed to be localised to the junctions of epithelial cell membranes with nerve endings, both in normal and pSS glands. The results indicate that anti-m3AChR antibodies bind to the receptors in the salivary glands. |
| Databáze: | OpenAIRE |
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