Changes of soluble CD40 ligand in the progression of acute myocardial infarction associate to endothelial nitric oxide synthase polymorphisms and vascular endothelial growth factor but not to platelet CD62P expression

Autor: Patrícia Napoleão, Catarina Ramos, Maria Céu Monteiro, Mafalda Selas, Rui Cruz Ferreira, Miguel Mota Carmo, Teresa Pinheiro, Ana Maria Viegas-Crespo, Luís B.P. Cabral, Carlota Saldanha, Maria Begoña Criado
Přispěvatelé: Repositório da Universidade de Lisboa
Rok vydání: 2015
Předmět:
Blood Platelets
Male
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Myocardial Infarction/pathology
P-selectin
Nitric Oxide Synthase Type III
Angiogenesis
Recombinant Fusion Proteins
Myocardial Infarction
HSM CAR
Myocardial Infarction/enzymology
Vascular Endothelial Growth Factor A/genetics
chemistry.chemical_compound
P-Selectin/genetics
Physiology (medical)
Internal medicine
Medicine
Humans
Platelet
Platelet activation
Endothelial dysfunction
Recombinant Fusion Proteins/blood
Aged
Myocardial Infarction/metabolism
Polymorphism
Genetic
business.industry
Biochemistry (medical)
Public Health
Environmental and Occupational Health
General Medicine
Middle Aged
medicine.disease
Vascular endothelial growth factor
Vascular endothelial growth factor B
Vascular endothelial growth factor A
P-Selectin
Endocrinology
chemistry
Immunology
Nitric Oxide Synthase Type III/genetics
Disease Progression
Female
Blood Platelets/metabolism
business
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação
ISSN: 1878-1810
Popis: © 2015 Elsevier Inc. All rights reserved.
Reported in vitro data implicated soluble CD40 ligand (sCD40L) in endothelial dysfunction and angiogenesis. However, whether sCD40L could exert that influence in endothelial dysfunction and angiogenesis after injury in acute myocardial infarction (AMI) patients remains unclear. In the present study, we evaluated the association of sCD40L with markers of platelet activation, endothelial, and vascular function during a recovery period early after AMI. To achieve this goal, the time changes of soluble, platelet-bound, and microparticle-bound CD40L levels over 1 month were assessed in AMI patients and correlated with endothelial nitric oxide synthase (eNOS) polymorphisms, vascular endothelial growth factor (VEGF) concentrations, and platelet expression of P-selectin (CD62P). The association of soluble form, platelet-bound, and microparticle-bound CD40L with CD62P expression on platelets, a marker of platelet activation, was also assessed to evaluate the role of CD40L in the thrombosis, whereas the association with eNOS and VEGF was to evaluate the role of CD40L in vascular dysfunction. This work shows for the first time that time changes of sCD40L over 1 month after myocardial infarct onset were associated with G894T eNOS polymorphism and with the VEGF concentrations, but not to the platelet CD62P expression. These results indicate that, in terms of AMI pathophysiology, the sCD40L cannot be consider just as being involved in thrombosis and inflammation but also as having a relevant role in vascular and endothelial dysfunction.
The work was financially supported by Fundação para a Ciência e Tencnologia (SFRM/BPD/6308/2009) and by Liga dos Amigos do Hospital de Santa Marta.
Databáze: OpenAIRE
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