Characterization of Haemophilus influenzae type b fimbriae
| Autor: | P M Mendelman, Arnold L. Smith, M A Schoenborn, K D Mack, Terrence L. Stull, J E Haas |
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| Rok vydání: | 1984 |
| Předmět: |
Haemophilus Infections
Immunology Fimbria Mannose Biology medicine.disease_cause Microbiology Haemophilus influenzae chemistry.chemical_compound medicine Humans Gel electrophoresis Strain (chemistry) Erythrocyte Membrane Adhesiveness Culture Media Titer Transformation (genetics) Microscopy Electron Infectious Diseases Hemagglutinins chemistry Fimbriae Bacterial Parasitology Bacterial outer membrane Bacterial Outer Membrane Proteins Research Article |
| Zdroj: | Infection and immunity. 46(3) |
| ISSN: | 0019-9567 |
| Popis: | We confirmed that the fimbriae of Haemophilus influenzae type b conferred hemagglutinating activity (HA) towards human erythrocytes, and erythrocytes of certain other species. Most (17/25) cerebrospinal fluid isolates lacked detectable HA on direct testing, but selective enrichment for fimbriation (f+) indicated that 22 of 25 strains could produce these surface structures. HA was unchanged from pH 4.5 to 9.5 and was not inhibited by mannose or certain other simple sugars. The HA titer of a suspension of three f+ strains was slightly decreased at 50 degrees C; HA was lost by heating at 60 degrees C for 3 min. Growth on a variety of solid and liquid media and under differing degrees of oxygenation did not change the HA titer of a suspension of three f+ strains. Fimbriation was not lost on repeated subculture. Wild-type fimbriated strains, and those derived by transformation, did not contain detectable plasmid DNA. Transformation of a strain lacking fimbriae to f+ was associated with the appearance of an outer membrane protein of 24 kilodaltons. This protein was purified from one strain to homogeneity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis by selective detergent solubilization and ammonium sulfate fractionation. Colonization capacity was equivalent with an isogenic untypable strain lacking or possessing fimbriae. Fimbriae of type b H. influenzae possess characteristics similar to those structures on other gram-negative bacteria; their role in cell physiology or pathogenesis of invasive disease is unknown. |
| Databáze: | OpenAIRE |
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