Analysis of sodium dodecyl sulfate-stable cell wall aminopeptidases in strains of viridans streptococci
| Autor: | Lars E. Linder, Marie-Louise Sund, Helena Lönnies |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Immunoelectrophoresis
Cross Reactions Microbiology Aminopeptidase Aminopeptidases Cell wall chemistry.chemical_compound Species Specificity Cell Wall Enzyme Stability Genetics medicine Humans Sodium dodecyl sulfate Molecular Biology Gel electrophoresis Mouth medicine.diagnostic_test biology Sodium Dodecyl Sulfate Streptococcus biology.organism_classification Streptococcaceae Streptococcus mutans Molecular Weight Biochemistry chemistry Viridans streptococci Chromatography Gel Electrophoresis Polyacrylamide Gel Immunoelectrophoresis Two-Dimensional |
| Zdroj: | FEMS microbiology letters. 143(1) |
| ISSN: | 0378-1097 |
| Popis: | Cell wall extracts from nine strains of viridans streptococci representing five species were analyzed for aminopeptidases. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis in combination with zymogram procedures revealed a common pattern of aminopeptidases in six strains, all having enzyme bands at 89 and 45 kDa. Three strains had an additional band at 200 kDa. Crossreactivity between aminopeptidases of all active strains was shown with crossed immunoelectrophoresis. Strains of Streptococcus mutans and S. sobrinus were without detectable cell wall aminopeptidases. |
| Databáze: | OpenAIRE |
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