Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells
| Autor: | A.J. Grant, Wanda B. Mackinnon, Rebecca L. Hancock, Peter Russell, Carolyn E. Mountford, Marlen Dyne |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Mutant
Drug Resistance Mice Nude Soft Tissue Neoplasms CHO Cells Drug resistance Biology Pathology and Forensic Medicine Malignant transformation Mice In vivo Cricetinae Endopeptidases Animals Neoplasm Invasiveness ATP Binding Cassette Transporter Subfamily B Member 1 Chromosome Aberrations Genetics Membrane Glycoproteins Chinese hamster ovary cell Wild type In vitro Cell Transformation Neoplastic Cell culture Cancer research Sarcoma Experimental Carrier Proteins |
| Zdroj: | ResearcherID |
| ISSN: | 0031-3025 |
| DOI: | 10.3109/00313029309066588 |
| Popis: | Chinese hamster ovary (CHO) cell lines are a very popular cell model for a wide range of studies but are often misused experimentally as a substitute for normal cells. Although CHO was originally derived from normal tissue, the cell lines studied here, including the parental wild type, have many characteristics which indicate that they have undergone malignant transformation. Biological properties associated with malignancy were investigated in this study on wild type CHO cells and 4 drug resistant sublines, EOT, Col R-22, Pod R11-6, and Vin R-1. We report evidence of tumorigenicity in experimental animals, invasive capacity, in vivo and in vitro, protease release by 2 of the cell lines, features related to drug resistance in the mutant sublines, and numerical and structural chromosomal abnormalities. |
| Databáze: | OpenAIRE |
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