Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (JcDGAT1 and JcDGAT2) Enhances Oil Production in the Woody Perennial Biofuel Plant Jatropha curcas
| Autor: | Chuan-Jia Xu, Tian-Tian Zhang, Zeng-Fu Xu, Qiantang Fu, Yan-Bin Tao, Ronghua Xu, Huiying He |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Perennial plant Heterologous Plant Science Genetically modified crops Biology 01 natural sciences Article 03 medical and health sciences Dry weight Food science Ecology Evolution Behavior and Systematics chemistry.chemical_classification J. curcas Ecology Botany Fatty acid food and beverages biology.organism_classification 030104 developmental biology Enzyme chemistry seed kernels Biofuel QK1-989 TAG oil content lipids (amino acids peptides and proteins) fatty acid leaves Jatropha curcas 010606 plant biology & botany |
| Zdroj: | Plants Plants, Vol 10, Iss 699, p 699 (2021) Volume 10 Issue 4 |
| ISSN: | 2223-7747 |
| Popis: | Diacylglycerol acyltransferase (DGAT) is the only enzyme that catalyzes the acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol (DAG) to form triacylglycerol (TAG). The two main types of DGAT enzymes in the woody perennial biofuel plant Jatropha curcas, JcDGAT1 and JcDGAT2, were previously characterized only in heterologous systems. In this study, we investigated the functions of JcDGAT1 and JcDGAT2 in J. curcas.JcDGAT1 and JcDGAT2 were found to be predominantly expressed during the late stages of J. curcas seed development, in which large amounts of oil accumulated. As expected, overexpression of JcDGAT1 or JcDGAT2 under the control of the CaMV35S promoter gave rise to an increase in seed kernel oil production, reaching a content of 53.7% and 55.7% of the seed kernel dry weight, respectively, which were respectively 25% and 29.6% higher than that of control plants. The increase in seed oil content was accompanied by decreases in the contents of protein and soluble sugars in the seeds. Simultaneously, there was a two- to four-fold higher leaf TAG content in transgenic plants than in control plants. Moreover, by analysis of the fatty acid (FA) profiles, we found that JcDGAT1 and JcDGAT2 had the same substrate specificity with preferences for C18:2 in seed TAGs, and C16:0, C18:0, and C18:1 in leaf TAGs. Therefore, our study confirms the important role of JcDGAT1 and JcDGAT2 in regulating oil production in J. curcas. |
| Databáze: | OpenAIRE |
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