Monoclonal Antibody Recognizing Pre-S(2) Epitope of Hepatitis B Virus: Characterization of Pre-S(2) Epitope and Anti-Pre-S(2) Antibody
| Autor: | Eliane Sobczak, Yamina Lazizi, Agata Budkowska, M.-M. Riottot, Jacques Pillot, Christian Bréchot, Pascal Dubreuil, Marie-Anne Petit |
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| Rok vydání: | 1986 |
| Předmět: |
Hepatitis B virus
HBsAg medicine.drug_class Hepatitis B virus DNA polymerase Radioimmunoassay medicine.disease_cause Monoclonal antibody Epitope Epitopes Mice Virology medicine Animals Humans Mice Inbred BALB C Hepatitis B Surface Antigens biology Antibodies Monoclonal Hepatitis B medicine.disease Molecular biology Recombinant Proteins Infectious Diseases HBeAg Acute Disease Chronic Disease biology.protein Antibody |
| Zdroj: | Journal of Medical Virology. 20:111-125 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.1890200204 |
| Popis: | A hybrid cell line producing monoclonal antibodies recognizing an epitope encoded by the pre-(S)2 region of hepatitis B virus (HBV) genome was obtained by fusion of mouse myeloma cells with lymphocytes from mice immunized with HBV. The monoclonal antibody Mo-F124 secreted from the hybrid line reacted with the pre-S(2) epitope expressed on the surface of both viral and recombinant HBsAg particles–pre-S(2) and S gene product–localised on 34 kD glycoprotein of the viral envelope. The pre-S(2) epitope was sensitive to digestion with V8 protease from Staphylucoccus aureus. The enzyme abolished reactivity with Mo-F124 and polymerized human serum albumin (pHSA) binding activity of recombinant particles. Mo-F124 antibody was used to develop highly sensitive radioimmunoassays for determination of pre-S(2) epitope and anti-pre-S(2) antibody in sera of hepatitis B patients. Detection of a pre-S(2) epitope by the monoclonal antibody-based assay in the early phase of acute HBV infection correlated well with the presence of markers of active viral replication (HBeAg, HBV DNA). The appearance of anti-pre-S(2) antibody, usually in the third month after onset of symptoms, was followed by elimination of circulating HBsAg and seroconversion to anti-HBs in all tested cases of uncomplicated acute hepatitis followed by recovery. Anti-pre-S(2) response was not observed in patients with chronic hepatitis B or acute HBV infection progressing to chronic disease. The observed correlation of anti-pre-S(2) response with recovery suggests that the pre-S(2) epitope may represent one of the epitopes inducing antibodies that neutralize the hepatitis B virus. |
| Databáze: | OpenAIRE |
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