Tumor necrosis factor-alpha and -beta upregulate the levels of osteoprotegerin mRNA in human osteosarcoma MG-63 cells
| Autor: | Sverker Ljunghall, Olle Vidal, Helena Brändström, Claes Ohlsson, Kenneth B. Jonsson, Östen Ljunggren |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Stromal cell Time Factors CD30 Transcription Genetic Biophysics Receptors Cytoplasmic and Nuclear Bone Neoplasms Biochemistry Polymerase Chain Reaction Receptors Tumor Necrosis Factor Osteoprotegerin Downregulation and upregulation Osteoclast Internal medicine medicine Tumor Cells Cultured Humans RNA Messenger Molecular Biology Lymphotoxin-alpha Glycoproteins Messenger RNA Osteosarcoma Chemistry Tumor Necrosis Factor-alpha Cell Biology medicine.disease Up-Regulation Kinetics medicine.anatomical_structure Endocrinology Tumor necrosis factor alpha |
| Zdroj: | Biochemical and biophysical research communications. 248(3) |
| ISSN: | 0006-291X |
| Popis: | Osteoprotegerin (OPG) is a recently cloned soluble member of the tumor necrosis factor receptor family. OPG has been shown to inhibit osteoclast recruitment by binding to OPG-ligand, an osteoclast differentiating factor on osteoblastic stromal cells, thereby blocking osteoclastogenesis. In this report we have examined the effect of tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta) on OPG mRNA levels in the human osteosarcoma cell line MG-63. We demonstrate that both TNF-alpha and TNF-beta dose- and time-dependently upregulate the mRNA levels of OPG. The effect is significant at and above 5 pM of TNF-alpha and 1 pM of TNF-beta. The stimulatory effect on OPG mRNA levels in MG-63 cells was detected after 2 hrs of incubation with TNF-alpha or TNF-beta. These data demonstrate that the expression of OPG in osteoblasts, with subsequent effects on osteoclastogenesis, is regulated by TNFs. |
| Databáze: | OpenAIRE |
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