Perinatal cocaine exposure reduces myocardial norepinephrine transporter function in the neonatal rat
| Autor: | Yejun Zhao, Lena Sun |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Sympathetic nervous system Time Factors Tyrosine 3-Monooxygenase Blotting Western Tritium Toxicology Reuptake Rats Sprague-Dawley Norepinephrine Cellular and Molecular Neuroscience Cocaine Developmental Neuroscience Pregnancy Internal medicine medicine Animals Vasoconstrictor Agents Analysis of Variance Norepinephrine Plasma Membrane Transport Proteins Symporters biology Perinatal Exposure Tyrosine hydroxylase business.industry Myocardium Age Factors Brain Gene Expression Regulation Developmental Heart Rats Endocrinology medicine.anatomical_structure Animals Newborn Norepinephrine transporter Prenatal Exposure Delayed Effects biology.protein Catecholamine Gestation Female business medicine.drug |
| Zdroj: | Neurotoxicology and Teratology. 26:443-450 |
| ISSN: | 0892-0362 |
| DOI: | 10.1016/j.ntt.2004.01.011 |
| Popis: | Norepinephrine transporter (NET) mediates the active removal of norepinephrine (NE) released from sympathetic nerve terminals via reuptake, and NET function and expression can be regulated by cocaine. NET expression and its regulation by cocaine in the developing sympathetic nervous system during early postnatal period, however, have not been examined. We quantified immunodetectable NET protein expression in the neonatal rat heart to examine the developmental pattern of myocardial NET during the first 2 weeks after birth. To assess sympathetic innervations, we simultaneously quantified the expression of myocardial tyrosine hydroxylase (TH). Timed pregnant rats received daily intragastric treatment with saline (CTL) or cocaine at 60 mg/kg (Coc) from Gestational Day 2 until parturition. After birth, nursing mothers continued to receive the same treatment. The expression of myocardial TH and NET in neonatal rats were then studied at 1 day (Postnatal Day 1, PD1), 7 days (PD7) or 14 days (PD14) of age. We observed a similar age-dependent increase in the expression for myocardial NET and TH during the first 2 weeks of postnatal life, in both CTL and Coc animals. While myocardial TH was significantly up-regulated following perinatal cocaine exposure, no significant change in immunodetectable myocardial NET protein was evident. To further examine whether NET function might be affected by perinatal cocaine exposure, we performed NE uptake in myocardial membranes from PD14 CTL and Coc rats. We found that NE uptake was reduced at PD14 in the cocaine-treated group. Our results indicate that myocardial NET and TH are both developmentally regulated. Furthermore, our results indicate that perinatal exposure to cocaine did not change NET protein expression but impaired myocardial NET function in the neonatal rat. |
| Databáze: | OpenAIRE |
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