Biofunctionalization with a TGFβ-1 inhibitor peptide in the osseointegration of synthetic bone grafts : An in vivo study in beagle dogs
| Autor: | Pablo Galindo-Moreno, Monica Ortiz-Hernandez, Andrea Cirera, Maria Cristina Manzanares, Javier Gil, Pablo Sevilla |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ossos -- Cirurgia
Gossos Empelts ossis Peptide Prosthesis 02 engineering and technology Beagle lcsh:Technology 0302 clinical medicine TGF-β1 General Materials Science Pròtesis Experimentació animal lcsh:QC120-168.85 chemistry.chemical_classification Bone growth Chemistry Biomaterial 021001 nanoscience & nanotechnology Prótesis Pèptids 0210 nano-technology lcsh:TK1-9971 Scanning electron microscopy scanning electron microscopy Histomorphometrical analysis histomorphometrical analysis Bone grafting Cirurgia dental Biofunctionalization Osseointegration Article 03 medical and health sciences Dogs In vivo Synthetic bone graft Teixit ossi Bone lcsh:Microscopy Perros Dental alveolus lcsh:QH201-278.5 lcsh:T biofunctionalization tgf-β1 osseointegration TGFbeta- 1 030206 dentistry Huesos -- Cirugía Bones--Surgery lcsh:TA1-2040 Dental surgery Animal experimentation synthetic bone graft lcsh:Descriptive and experimental mechanics Implant lcsh:Electrical engineering. Electronics. Nuclear engineering Peptides lcsh:Engineering (General). Civil engineering (General) Biomedical engineering |
| Zdroj: | Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Materials, Vol 12, Iss 19, p 3168 (2019) Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona Materials Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Volume 12 Issue 19 Digibug. Repositorio Institucional de la Universidad de Granada |
| Popis: | Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial&mdash one untreated and another submitted to biofunctionalization with a TGF-&beta 1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial&rsquo s granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial&rsquo s granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant&rsquo s osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials. |
| Databáze: | OpenAIRE |
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