MluI site-dependent transcriptional regulation of the Candida albicans dUTPase gene
| Autor: | Robert H. Haynes, Ronald E. Pearlman, Jens Looser, Evan M. McIntosh |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA Replication
Transcription Genetic Genes Fungal Molecular Sequence Data Restriction Mapping Biology Immunodeficiency Virus Feline Polymerase Chain Reaction Gene Expression Regulation Enzymologic Bacterial Proteins Solanum lycopersicum Transcription (biology) DUTP pyrophosphatase Gene Expression Regulation Fungal Candida albicans Genetics Transcriptional regulation Escherichia coli Humans Amino Acid Sequence Cloning Molecular Pyrophosphatases Deoxyribonucleases Type II Site-Specific Gene DNA Primers Base Sequence Sequence Homology Amino Acid DNA replication RNA Fungal General Medicine biology.organism_classification Blotting Northern Molecular biology Corpus albicans Recombinant Proteins Complementation |
| Zdroj: | Current genetics. 26(5-6) |
| ISSN: | 0172-8083 |
| Popis: | The Candida albicans dUTP pyrophosphatase (dUTPase) gene DUT1 has been isolated by genetic complementation in S. cerevisiae. It was found to encode a 17-kDa protein similar in amino-acid sequence to dUTPases isolated from other systems. The gene was adapted for expression in E. coli and yielded a soluble and highly-active enzyme which is easily purified. The 5' flanking sequence of DUT1 contains an MluI site typical of MCB cell-cycle-dependent UAS elements of budding and fission yeast. We found the gene to be cell-cycle-regulated when expressed in S. cerevisiae, and deletion of the MluI site resulted in a large reduction of DUT1 transcription in C. albicans. These results suggest that MCB elements are functionally conserved in this pathogenic fungus. Based on the vital role that dUTPase plays in DNA replication, the C. albicans enzyme may be a potentially useful target for the development of novel anti-fungal compounds. |
| Databáze: | OpenAIRE |
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