High incidence of genotypic variance between sequential herpes simplex virus type 2 isolates from HIV-1-seropositive patients with recurrent genital herpes
| Autor: | R. Wim Roest, Willem I. van der Meijden, Marius Kant, Georges M. G. M. Verjans, Albert D. M. E. Osterhaus, Jeroen Maertzdorf |
|---|---|
| Přispěvatelé: | Dermatology, Virology |
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Sexually transmitted disease Genotype Herpesvirus 2 Human viruses HIV Infections Biology medicine.disease_cause Polymerase Chain Reaction Herpesviridae Virus law.invention SDG 3 - Good Health and Well-being Predictive Value of Tests Recurrence law medicine Humans Immunology and Allergy Genotyping Polymerase chain reaction Netherlands Herpes Genitalis Incidence Virology Infectious Diseases Herpes simplex virus Superinfection DNA Viral HIV-1 Female |
| Zdroj: | Journal of Infectious Diseases, 194(8), 1115-1118. Oxford University Press |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/507683 |
| Popis: | We developed a polymerase chain reaction (PCR) method, based on strain-to-strain variation of DNA repeats in the herpes simplex virus type 2 (HSV-2) genes US1 and US12, to genotype HSV-2 strains and determine the incidence and risk factors associated with HSV-2 superinfection in patients with recurrent genital herpes (RGH). Forty-seven (92%) of 51 unrelated HSV-2 isolates could be distinguished. Genotyping of sequential HSV-2 isolates showed a different genotype in all of the 11 human immunodeficiency virus type 1 (HIV-1)-seropositive patients with RGH, compared with 1 of the 8 HIV-1-seronegative patients with RGH. The PCR approach developed distinguishes unrelated HSV-2 strains efficiently and indicated a high incidence of genotype variance between sequential HSV-2 isolates of HIV-1-seropositive patients with RGH. |
| Databáze: | OpenAIRE |
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