Cell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells
| Autor: | Maude Gluais, Yoann Torres, Nicolas L'Heureux, Laure Magnan, Sylvie Rey, Nicolas Da Silva, Agathe Grémare, Fabien Kawecki |
|---|---|
| Přispěvatelé: | Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pôle d’Odontologie et de Santé Buccale [CHU Bordeaux], CHU Bordeaux [Bordeaux], Chassande, Olivier |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
[SDV]Life Sciences [q-bio]
medicine.medical_treatment large animal Cell Biomedical Engineering Skin fibroblasts Medicine (miscellaneous) Human skin Biomaterials Extracellular matrix lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine cell-assembled extracellular matrix vascular tissue-engineering medicine lcsh:QD415-436 Fibroblast 030304 developmental biology 0303 health sciences Chemistry Insulin Growth factor Translation (biology) In vitro Cell biology [SDV] Life Sciences [q-bio] medicine.anatomical_structure 030220 oncology & carcinogenesis Original Article interspecies differences |
| Zdroj: | Journal of Tissue Engineering, Vol 12 (2021) Journal of Tissue Engineering and Regenerative Medicine Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2021, 12, pp.204173142097832. ⟨10.1177/2041731420978327⟩ Journal of Tissue Engineering |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | International audience; We have created entirely biological tissue-engineered vascular grafts (TEVGs) using sheets of cell-assembled extracellular matrix (CAM) produced by human fibroblasts in vitro. A large animal TEVG would allow long-term pre-clinical studies in a clinically relevant setting (graft size and allogeneic setting). Therefore, canine, porcine, ovine, and human skin fibroblasts were compared for their ability to form CAM sheets. Serum sourcing greatly influenced CAM production in a species-dependent manner. Ovine cells produced the most homogenous and strongest animal CAM sheets but remained ≈3-fold weaker than human sheets despite variations of serum, ascorbate, insulin, or growth factor supplementations. Key differences in cell growth dynamics, tissue development, and tissue architecture and composition were observed between human and ovine. This study demonstrates critical species-to-species differences in fibroblast behavior and how they pose a challenge when attempting to substitute animal cells for human cells during the development of tissueengineered constructs that require long-term cultures. |
| Databáze: | OpenAIRE |
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