Immunogenicity and efficacy of DNA vaccines encoding influenza A proteins in aged mice
| Autor: | Corrille M. DeWitt, A. Friedman, M.A. Liu, A.M. Ward, Jeffrey B. Ulmer, J.J. Donnelly, Bradley S. Bender, Robert Cottey, S F Taylor |
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| Rok vydání: | 1998 |
| Předmět: |
Cellular immunity
Aging Influenza vaccine viruses Orthomyxoviridae Hemagglutinin Glycoproteins Influenza Virus medicine.disease_cause Virus DNA vaccination Mice Viral Proteins Influenza A virus medicine Vaccines DNA Animals Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health virus diseases biology.organism_classification Virology Vaccination Infectious Diseases Nucleoproteins Immunology Molecular Medicine Female T-Lymphocytes Cytotoxic |
| Zdroj: | Vaccine. 16(18) |
| ISSN: | 0264-410X |
| Popis: | Influenza is a leading cause of morbidity and mortality in older persons. The current influenza vaccine is only modestly successful, in part because of an age-related decline in immunogenicity and also because it induces only type-specified immunity. To overcome this, we evaluated DNA vaccines encoding A/PR8/34 haemagglutinin (HA) and nucleoprotein (NP) in young and aged BALB/c mice. Control mice were given formalin-inactivated A/PR8/34, control DNA, or a non-lethal dose of PR8. Aged mice given HA DNA developed slightly lower anti-HA serum antibodies than young mice; however, both young and aged mice were protected from a homotypic PR8 challenge. Following vaccination with NP DNA, both young and aged mice developed anti-NP bulk cytotoxic T-lymphocyte (CTL) activity and pCTL frequency similar to control animals. When challenged with a low dose of A/HK/68 (H3N2) influenza virus, both young mice and aged mice showed significant protection as measured by inhibition of weight loss. When challenged with a relatively high dose of A/HR/68 (H3N2) influenza virus, however, the anti-NP vaccine only partially protected young mice and failed to protect aged mice. These data demonstrate that DNA-based vaccines are immunogenic in aged animals, but suggest that factors other than the age-related decline in CTL activity also contribute to the increased morbidity and mortality of influenza in the elderly. |
| Databáze: | OpenAIRE |
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