Hexokinase regulates kinetics of glucose transport and expression of genes encoding hexose transporters in Saccharomyces cerevisiae
| Autor: | Arthur L. Kruckeberg, Carlos Gancedo, Jasper A. Diderich, Karel van Dam, Thomas Petit |
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| Přispěvatelé: | Molecular Microbial Physiology (SILS, FNWI), Molecular Cell Physiology |
| Rok vydání: | 2000 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Monosaccharide Transport Proteins Saccharomyces cerevisiae Glucose Transport Proteins Facilitative Biology Microbiology Saccharomyces Fungal Proteins chemistry.chemical_compound Gene Expression Regulation Fungal Hexokinase Hexose Molecular Biology Derepression Hexose transport chemistry.chemical_classification Glucose transporter Membrane Proteins Biological Transport biology.organism_classification Kinetics Glucose Eukaryotic Cells chemistry Biochemistry Schizosaccharomyces pombe |
| Zdroj: | Journal of Bacteriology, 182, 6815-6818. American Society for Microbiology Petit, T, Diderich, J A, Kruckeberg, A L, Gancedo, C & van Dam, K 2000, ' Hexokinase regulates kinetics of glucose transport and expression of genes encoding hexose transporters in Saccharomyces cerevisiae. ', Journal of Bacteriology, vol. 182, pp. 6815-6818 . https://doi.org/10.1128/JB.182.23.6815-6818.2000 |
| ISSN: | 0021-9193 |
| DOI: | 10.1128/JB.182.23.6815-6818.2000 |
| Popis: | Glucose transport kinetics and mRNA levels of different glucose transporters were determined in Saccharomyces cerevisiae strains expressing different sugar kinases. During exponential growth on glucose, a hxk2 null strain exhibited high-affinity hexose transport associated with an elevated transcription of the genes HXT2 and HXT7 , encoding high-affinity transporters, and a diminished expression of the HXT1 and HXT3 genes, encoding low-affinity transporters. Deletion of HXT7 revealed that the high-affinity component is mostly due to HXT7 ; however, a previously unidentified very-high-affinity component ( K m = 0.19 mM) appeared to be due to other factors. Expression of genes encoding hexokinases from Schizosaccharomyces pombe or Yarrowia lipolytica in a hxk1 hxk2 glk1 strain prevented derepression of the high-affinity transport system at high concentrations of glucose. |
| Databáze: | OpenAIRE |
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